Phytoreovirus

VIRION

Non enveloped, icosahedral, non-turreted virion with a double capsid structure, about 70 nm in diameter. The outer capsid has a T=13 icosahedral symmetry, the inner capsid a T=2* icosahedral symmetry. Each double-layered capsid consists of 260 trimers of the major outer capsid P8 protein and 60 dimers of the inner capsid P3 protein.

GENOME

Segmented linear dsRNA genome. Contains 12 segments coding for 15 proteins. Segment 11 has two in-frame codons resulting in two ORFs. Segment 12 has two additional overlapping ORFs. Segments size range from 1066 to 4423 bp. Genome total size is about 26 kb (WTV).

GENE EXPRESSION

The dsRNA genome is never completely uncoated, to prevent activation of antiviral state by the cell in response to dsRNA. The viral polymerase synthesizes a capped mRNA from each dsRNA segment. This capped mRNA is translocated to the cell cytoplasm where it is translated. RDV expresses an additional protein by leaky scanning in the nsp12 gene.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Early transcription of the dsRNA genome by viral polymerase occurs inside this sub-viral particle (naked core), so that dsRNA is never exposed to the cytoplasm.
  3. Full-length plus-strand transcripts from each of the dsRNA segments are synthesized. These plus-strand transcripts are used as templates for translation.
  4. Viral proteins and genomic RNAs aggregates in cytoplasmic viral factories.
  5. (+)RNAs are encapsidated in a sub-viral particle, in which they are transcribed to give RNA (-) molecules with which they become base-paired to produce dsRNA genomes.
  6. The capsid is assembled on the sub-viral particle.
  7. Mature virions are released presumably following cell death and associated breakdown of host plasma membrane.

Host-virus interaction

Suppression of host silencing-mediated antiviral defense

Rice gall dwarf virus Pns11 and Pns12 proteins as well as rice dwarf virus Pns10 suppress host cell RNA silencing

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Matching UniProtKB/Swiss-Prot entries

56 entries grouped by protein (browse by keywords)

All proteins are shown (view only 25 complete proteome entries)

4 entries

Putative mRNA-capping enzyme P5 (Structural protein 5) (mRNA guanylyltransferase P5) (EC 2.7.7.50)

Select_allDeselect_all
MCE_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain

Putative mRNA-capping enzyme P5 (Structural protein 5) (mRNA guanylyltransferase) (EC 2.7.7.50)

MCE_RGDV
Rice gall dwarf virus (RGDV) reference strain
MCE_RDVA
Rice dwarf virus (isolate Akita) (RDV)

Putative mRNA-capping enzyme P5 (Structural protein 5) (mRNA guanylyltransferase) (EC 2.7.7.50)

MCE_WTV
Wound tumor virus (WTV)

3 entries

Movement protein (Non-structural protein 6) (Pns6)

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MVP_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
MVP_RGDV
Rice gall dwarf virus (RGDV) reference strain

Movement protein (Non-structural protein 7) (Pns7)

MVP_RDVA
Rice dwarf virus (isolate Akita) (RDV)

5 entries

RNA-binding protein (Non-structural protein 12) (Pns12)

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NSP11_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain

RNA-binding protein (Non-structural protein 11) (Pns11)

NSP11_RGDV
Rice gall dwarf virus (RGDV) reference strain
NSP11_RDVA
Rice dwarf virus (isolate Akita) (RDV)

RNA-binding protein (Non-structural protein 11) (Pns11)

NSP11_WTV
Wound tumor virus (WTV)
NSP11_WTVNJ
Wound tumor virus (strain NJ) (WTV)

4 entries

Non-structural protein 12A (Pns12A)

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NSP12_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
NSP12_RGDV
Rice gall dwarf virus (RGDV) reference strain

Non-structural protein 9 (Pns9)

NSP12_RDVA
Rice dwarf virus (isolate Akita) (RDV)
NSP12_RDVO
Rice dwarf virus (isolate O) (RDV)

4 entries

Non-structural protein 4 (Pns4)

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NSP4_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
NSP4_RGDV
Rice gall dwarf virus (RGDV) reference strain
NSP4_RDVA
Rice dwarf virus (isolate Akita) (RDV)
NSP4_WTV
Wound tumor virus (WTV)

1 entry

Protein OP-ORF

OPORF_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain

3 entries

Minor outer capsid protein P2

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P2_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
P2_RGDV
Rice gall dwarf virus (RGDV) reference strain
P2_RDVO
Rice dwarf virus (isolate O) (RDV)

3 entries

Outer capsid protein P3 (Core protein P3)

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P3_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
P3_RGDV
Rice gall dwarf virus (RGDV) reference strain
P3_RDVA
Rice dwarf virus (isolate Akita) (RDV)

6 entries

Protein P7 (55 kDa core protein)

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P7_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
P7_RGDV
Rice gall dwarf virus (RGDV) reference strain

Protein P7

P7_RDVA
Rice dwarf virus (isolate Akita) (RDV)
P7_RDVO
Rice dwarf virus (isolate O) (RDV)
P7_WTV
Wound tumor virus (WTV)

Protein P7 (60 kDa core protein)

P7_WTVNJ
Wound tumor virus (strain NJ) (WTV)

Protein P7 (60 kDa core protein)

6 entries

Outer capsid protein P8 (Structural protein P8)

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P8_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
P8_RGDV
Rice gall dwarf virus (RGDV) reference strain
P8_RDVA
Rice dwarf virus (isolate Akita) (RDV)
P8_RDVO
Rice dwarf virus (isolate O) (RDV)
P8_RDVS
Rice dwarf virus (isolate S) (RDV)
P8_WTV
Wound tumor virus (WTV)

7 entries

Minor outer capsid protein P9

Select_allDeselect_all
P9_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
P9_RGDV
Rice gall dwarf virus (RGDV) reference strain

Minor outer capsid protein P9 (Protein Pns10)

P9_RDV
Rice dwarf virus (RDV)
P9_RDVA
Rice dwarf virus (isolate Akita) (RDV)
P9_RDVO
Rice dwarf virus (isolate O) (RDV)
P9_WTV
Wound tumor virus (WTV)

Minor outer capsid protein P9 (Non-structural protein 11) (Pns11)

P9_WTVNJ
Wound tumor virus (strain NJ) (WTV)

Minor outer capsid protein P9 (Non-structural protein 11) (Pns11)

3 entries

RNA-directed RNA polymerase P1 (EC 2.7.7.48) (Replicase)

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RDRP_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
RDRP_RGDV
Rice gall dwarf virus (RGDV) reference strain
RDRP_RDVA
Rice dwarf virus (isolate Akita) (RDV)

1 entry

Non-structural protein Pns10

VP10_WTV
Wound tumor virus (WTV)

1 entry

Non-structural protein PNS7

VP7_WTV
Wound tumor virus (WTV)

5 entries

Suppressor of RNA-mediated gene silencing (Non-structural protein 10) (Pns10)

Select_allDeselect_all
VSR_RDVF
Rice dwarf virus (isolate Fujian) (RDV) reference strain
VSR_RGDV
Rice gall dwarf virus (RGDV) reference strain

Suppressor of RNA-mediated gene silencing (Non-structural protein 11) (Pns11)

VSR_RDVA
Rice dwarf virus (isolate Akita) (RDV)
VSR_RDVO
Rice dwarf virus (isolate O) (RDV)
VSR_WTV
Wound tumor virus (WTV)