Flavivirus

VIRION

Enveloped, spherical, about 50 nm in diameter. The surface proteins are arranged in an icosahedral-like symmetry. Mature virions contain two virus-encoded membrane proteins (M and E), while immature virions contain a membrane protein precursor.
Source: Zhang et al(Pubmed)

GENOME

Monopartite, linear, ssRNA(+) genome of of 10-11 kb. The genome 3’ terminus is not polyadenylated but forms a loop structure. The 5’ end has a methylated nucleotide cap (allows for translation) or a genome-linked protein (VPg).

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and the viral messenger RNA. The whole genome is translated in a polyprotein, which is processed co- and post-translationally by host and viral proteases.

REPLICATION

  1. Attachement of the viral envelope protein E to host receptors mediates internalization into the host cell by clathrin-mediated endocytosis.
  2. Fusion of virus membrane with host endosomal membrane. RNA genome is released into the cytoplasm.
  3. The positive-sense genomic ssRNA is translated into a polyprotein, which is cleaved into all structural and non structural proteins (to yield the replication proteins).
  4. Replication takes place at the surface of endoplasmic reticulum in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. Virus assembly occurs at the endoplasmic reticulum and seems to be facilitated by the viral ionic channel p7. The virion buds via the host ESCRT complexes at the endoplasmic reticulum, is transported to the Golgi apparatus, and then exits the cell via the secretory pathway.

Host-virus interaction

Adaptive immune response inhibition

Dengue NS1 protein inhibits host complement by binding C4 and reducing C4b deposition and C3 convertase activity. Through this mechanism, NS1 protects DENV from complement-dependent neutralization in solution .

Apoptosis modulation

Flaviviruses NS3 induces apoptosis through host caspases activation .

Autophagy modulation

Flaviviruses NS4A and NS4B induces autophagy signaling .

Innate immune response inhibition

Dengue virus blocks STAT2 .

Matching UniProtKB/Swiss-Prot entries

42 entries grouped by strain (browse by keywords)

1 entry

Dengue virus type 1 (strain Nauru/West Pac/1974) (DENV-1) reference strain


POLG_DEN1WGenome polyprotein

1 entry

Yellow fever virus (strain 17D vaccine) (YFV) reference strain


POLG_YEFV1Genome polyprotein

1 entry

Dengue virus type 1 (strain Brazil/97-11/1997) (DENV-1)


POLG_DEN1BGenome polyprotein

1 entry

Dengue virus type 1 (strain Singapore/S275/1990) (DENV-1)


POLG_DEN1SGenome polyprotein

1 entry

Dengue virus type 2 (isolate Thailand/0168/1979) (DENV-2)


POLG_DEN28Genome polyprotein

1 entry

Dengue virus type 2 (strain 16681-PDK53) (DENV-2)


POLG_DEN27Genome polyprotein

1 entry

Dengue virus type 2 (strain Jamaica/1409/1983) (DENV-2)


POLG_DEN2JGenome polyprotein

1 entry

Dengue virus type 2 (strain Peru/IQT2913/1996) (DENV-2)


POLG_DEN2QGenome polyprotein

1 entry

Dengue virus type 2 (strain Puerto Rico/PR159-S1/1969) (DENV-2)

1 entry

Dengue virus type 2 (strain Thailand/16681/1984) (DENV-2)


POLG_DEN26Genome polyprotein

1 entry

Dengue virus type 2 (strain Thailand/NGS-C/1944) (DENV-2)


POLG_DEN2NGenome polyprotein

1 entry

Dengue virus type 3 (strain China/80-2/1980) (DENV-3)


POLG_DEN3CGenome polyprotein

1 entry

Dengue virus type 3 (strain Martinique/1243/1999) (DENV-3)


POLG_DEN3MGenome polyprotein

1 entry

Dengue virus type 3 (strain Philippines/H87/1956) (DENV-3)


POLG_DEN3PGenome polyprotein

1 entry

Dengue virus type 3 (strain Singapore/8120/1995) (DENV-3)


POLG_DEN3IGenome polyprotein

1 entry

Dengue virus type 3 (strain Sri Lanka/1266/2000) (DENV-3)


POLG_DEN3SGenome polyprotein

1 entry

Dengue virus type 4 (strain Dominica/814669/1981) (DENV-4)


POLG_DEN4DGenome polyprotein

1 entry

Dengue virus type 4 (strain Philippines/H241/1956) (DENV-4)


POLG_DEN4PGenome polyprotein

1 entry

Dengue virus type 4 (strain Singapore/8976/1995) (DENV-4)


POLG_DEN4SGenome polyprotein

1 entry

Dengue virus type 4 (strain Thailand/0348/1991) (DENV-4)

1 entry

Dengue virus type 4 (strain Thailand/0476/1997) (DENV-4)


POLG_DEN4HGenome polyprotein

1 entry

Japanese encephalitis virus (strain Jaoars982)


POLG_JAEVJGenome polyprotein

1 entry

Japanese encephalitis virus (strain SA(v))


POLG_JAEV5Genome polyprotein

1 entry

Japanese encephalitis virus (strain SA-14)


POLG_JAEV1Genome polyprotein

1 entry

Kunjin virus (strain MRM61C)


POLG_KUNJMGenome polyprotein

1 entry

Langat virus (strain TP21)


POLG_LANVTGenome polyprotein

1 entry

Louping ill virus (Li)


POLG_LIVGenome polyprotein

1 entry

Mosquito cell fusing agent (CFA flavivirus)


POLG_MCFAGenome polyprotein

1 entry

Murray valley encephalitis virus (strain MVE-1-51) (MVEV)


POLG_MVEV5Genome polyprotein

1 entry

Tick-borne encephalitis virus (strain Hypr) (TBEV)


POLG_TBEVHGenome polyprotein

1 entry

Tick-borne encephalitis virus European subtype (strain Neudoerfl) (NEUV) (Neudoerfl virus)


POLG_TBEVWGenome polyprotein

1 entry

Tick-borne encephalitis virus Far Eastern subtype (strain Sofjin) (SOFV) (Sofjin virus)


POLG_TBEVSGenome polyprotein

1 entry

Tick-borne powassan virus (strain LB) (POWV) (Powassan virus)


POLG_POWVLGenome polyprotein

1 entry

West Nile virus (WNV)


POLG_WNVGenome polyprotein

1 entry

Yellow fever virus (isolate Angola/14FA/1971) (YFV)


POLG_YEFVNGenome polyprotein

1 entry

Yellow fever virus (isolate Ethiopia/Couma/1961) (YFV)


POLG_YEFVEGenome polyprotein

1 entry

Yellow fever virus (isolate Ivory Coast/1999) (YFV)


POLG_YEFVCGenome polyprotein

1 entry

Yellow fever virus (isolate Ivory Coast/85-82H/1982) (YFV)


POLG_YEFVIGenome polyprotein

1 entry

Yellow fever virus (isolate Uganda/A7094A4/1948) (YFV)


POLG_YEFVUGenome polyprotein

1 entry

Yellow fever virus (strain French neurotropic vaccine FNV) (YFV)


POLG_YEFVFGenome polyprotein

1 entry

Yellow fever virus (strain Ghana/Asibi/1927) (YFV)


POLG_YEFVAGenome polyprotein

1 entry

Yellow fever virus (strain Trinidad/TRINID79A/1979) (YFV)


POLG_YEFVTGenome polyprotein