Cuevavirus

Molecular biology

Filamentous, greatly variable up to 1400 nm in length. Diameter is about 80nm.

GENOME

Negative-stranded RNA linear genome, about 19 kb in size. Encodes for seven proteins.

GENE EXPRESSION

The viral RNA dependent RNA polymerase binds the encapsidated genome at the leader region, then sequentially transcribes each genes by recognizing start and stop signals flanking viral genes. mRNAs are capped and polyadenylated by the L protein during synthesis.

REPLICATION

CYTOPLASMIC

  1. Attachment to host receptors through GP glycoprotein mediates is endocytosed into vesicles in the host cell.
  2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid is released into the cytoplasm.
  3. Sequential transcription, viral mRNAs are capped and polyadenylated by polymerase stuttering in the cytoplasm.
  4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  5. The ribonucleocapsid interacts with the matrix protein under the plasma membrane, buds via the host ESCRT complexes from the plasma membrane, releasing the virion.

Host-virus interaction