Percavirus

VIRION

Enveloped, spherical to pleomorphic, 150-200 nm in diameter, T=16 icosahedral symmetry. Capsid consists of 162 capsomers and is surrounded by an amorphous tegument. Glycoproteins complexes are embeded in the lipid envelope.

GENOME

Monopartite, linear, dsDNA genome of about 180 kb. The genome contains terminal and internal reiterated sequences.

GENE EXPRESSION

Each viral transcript usually encodes a single protein and has a promoter/regulatory sequence, a TATA box, a transcription initiation site, a 5’ leader sequence of 30-300 bp (not translated), a 3’ nontranslated sequence of 10-30 bp, and a poly A signal. There are many gene overlaps. There are only few spliced genes. Some of the expressed ORFs are antisense to each other. Some ORFs can be accessed from more than one promoter. There are some non-coding genes.

REPLICATION

NUCLEAR

Lytic replication:

  1. Attachment of the viral glycoproteins to host receptors mediates endocytosis of the virus into the host cell.
  2. Fusion with the plasma membrane to release the core and the tegument proteins into the host cytoplasm.
  3. The capsid is transported to the nuclear pore where the viral DNA is released into the nucleus.
  4. Transcription of immediate early genes which promote transcription of early genes and protect the virus against innate host immunity.
  5. Transcription of early viral mRNA by host polymerase II, encoding proteins involved in replication of the viral DNA.
  6. A first round of circular genome amplification occurs by bidirectional replication
  7. Synthesis of linear concatemer copies of viral DNA by rolling circle.
  8. Transcription of late mRNAs by host polymerase II, encoding structural proteins.
  9. Assembly of the virus in nuclear viral factories and budding through the inner lamella of the nuclear membrane which has been modified by the insertion of herpes glycoproteins, throughout the Golgi and final release at the plasma membrane.

Latent replication : replication of circular viral episome in tandem with the host cell DNA using the host cell replication machinery.

Host-virus interaction

Adaptive immune response inhibition

The viral interleukin-10 homolog down-regulates the expression of the TAP1 gene (transporter associated with antigen processing), thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.

Apoptosis modulation

The EHV-2 v-FLIP protein (ORF E8) inhibits TNFRSF1A, TNFRSF6, TNFRSF10 and TNFRSF12 induced apoptosis.

Cell-cycle modulation

The UL24 protein induces a cell cycle arrest at G2/M transition through inactivation of the host cyclinB/cdc2 complex .

Splicing inhibition

Modulates the viral and host mRNA expression by exporting unspliced mRNA, thereby inducing alternative splicing.

Matching UniProtKB/Swiss-Prot entries

75 entries grouped by strain (browse by keywords)

All proteins are shown (view only 74 complete proteome entries)

74 entries

Equine herpesvirus 2 (strain 86/87) (EHV-2) reference strain

Select_all Deselect_all  
AN_EHV2Shutoff alkaline exonuclease (SOX) (EC 3.1.-.-)
BRLF1_EHV2Putative transcription activator BRLF1 homolog
CEP1_EHV2Cytoplasmic envelopment protein 1
CEP2_EHV2Cytoplasmic envelopment protein 2
CEP3_EHV2Cytoplasmic envelopment protein 3
CFLA_EHV2Viral CASP8 and FADD-like apoptosis regulator (v-CFLAR) (Viral FLICE-inhibitory ...
CVC1_EHV2Capsid vertex component 1
CVC2_EHV2Capsid vertex component 2
DNBI_EHV2Major DNA-binding protein
DPOL_EHV2DNA polymerase catalytic subunit (EC 2.7.7.7)
DUT_EHV2Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (EC 3.6.1.23) (dUTP ...
GB_EHV2Envelope glycoprotein B (gB)
GH_EHV2Envelope glycoprotein H (gH)
GL_EHV2Envelope glycoprotein L (gL)
GM_EHV2Envelope glycoprotein M (gM)
GN_EHV2Envelope glycoprotein N
HELI_EHV2DNA replication helicase (EC 3.6.4.-)
HEPA_EHV2DNA helicase/primase complex-associated protein (HEPA) (Primase-associated ...
IE63_EHV2Immediate-early phosphoprotein 57
IL10H_EHV2Viral interleukin-10 homolog (vIL-10)
ITP_EHV2Inner tegument protein
KITH_EHV2Thymidine kinase (EC 2.7.1.21)
LTP_EHV2Large tegument protein deneddylase (EC 3.4.19.12) (EC 3.4.22.-)
MCP_EHV2Major capsid protein (MCP)
NEC1_EHV2Nuclear egress protein 1
NEC2_EHV2Nuclear egress protein 2
PORTL_EHV2Portal protein
PRIM_EHV2DNA primase (EC 2.7.7.-)
RIR1_EHV2Ribonucleoside-diphosphate reductase large subunit (R1) (EC 1.17.4.1) ...
RIR2_EHV2Ribonucleoside-diphosphate reductase small subunit (EC 1.17.4.1) ...
SCAF_EHV2Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR)
SCP_EHV2Small capsomere-interacting protein
TEG7_EHV2Tegument protein UL51 homolog
TRM1_EHV2Tripartite terminase subunit 1
TRM2_EHV2Tripartite terminase subunit 2
TRM3_EHV2Tripartite terminase subunit 3 (EC 3.1.-.-) (Terminase large subunit)
TRX1_EHV2Triplex capsid protein 1
TRX2_EHV2Triplex capsid protein 2
TYSY_EHV2Thymidylate synthase (TS) (TSase) (EC 2.1.1.45)
UL24_EHV2Protein UL24 homolog
UL32_EHV2Packaging protein UL32 homolog
UL49_EHV2Uncharacterized gene 66 protein
UL79_EHV2Gene 18 protein
UL87_EHV2Gene 24 protein
UL91_EHV2Uncharacterized gene 30 protein
UL92_EHV2Gene 31 protein
UL95_EHV2Gene 34 protein
UL96_EHV2Gene 35 protein
UNG_EHV2Uracil-DNA glycosylase (UDG) (EC 3.2.2.27) (UNG)
VCLAP_EHV2CARD domain-containing protein E10
VG10_EHV2Uncharacterized gene 10 protein
VG11_EHV2Uncharacterized gene 11 protein
VG12_EHV2Protein E6A
VG13_EHV2Protein E6C
VG23_EHV2Uncharacterized gene 23 protein
VG27_EHV2Uncharacterized gene 27 protein
VG28_EHV2Uncharacterized gene 28 protein
VG36_EHV2Probable inactive protein kinase 38
VG45_EHV2Uncharacterized gene 45 protein
VG48_EHV2Tegument protein G48
VG49_EHV2Uncharacterized gene 49 protein
VG51_EHV2Putative C-type lectin protein 51
VG52_EHV2Gene 52 protein
VG58_EHV2Gene 58 protein
VG59_EHV2DNA polymerase processivity factor
VG74_EHV2G-protein coupled receptor 74
VG75_EHV2Probable membrane antigen 75 (Tegument protein)
VGE1_EHV2G-protein coupled receptor E1
VGE2_EHV2Uncharacterized gene E2 protein
VGE3_EHV2SUSHI domain-containing protein E3
VGE4_EHV2Uncharacterized gene E4 protein
VGE6_EHV2G-protein coupled receptor E6
VGE9_EHV2Uncharacterized gene E9 protein
VP03_EHV2Probable membrane antigen 3 (Tegument protein)

1 entry

Equine herpesvirus 2 (strain T400/3) (EHV-2)


IL10H_EHV2TViral interleukin-10 homolog (vIL-10)