Tombusvirus

VIRION

Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).

GENOME

Monopartite, linear, ssRNA(+) genome of 4-5.4 kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5’-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. The downstream genes encoding the movement protein, viral suppressor of RNA silencing and capsid proteins are translated from subgenomic RNAs.
Cap-independent translation is mediated possibly through the interaction between a 3’-cap-independent translation element and a 5’UTR stem-loop.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid and movement protein.
  7. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid protein, movement protein and viral suppressor of RNA silencing.
  8. Virus assembly in the cytoplasm.
  9. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

Tombusviruses encode P19/P20 protein acting as a suppressor of RNA silencing .

Matching UniProtKB/Swiss-Prot entries

32 entries grouped by strain (browse by keywords)

All proteins are shown (view only 11 complete proteome entries)

4 entries

Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain

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CAPSD_TBSVCCapsid protein (Coat protein) (p41)
MVP_TBSVCMovement protein (p22)
P19_TBSVCRNA silencing suppressor p19 (19 kDa symptom severity modulator)
RDRP_TBSVCRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

4 entries

Cucumber necrosis virus (CNV)

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CAPSD_CNVCapsid protein (Coat protein) (p41)
MVP_CNVMovement protein (p22)
P19_CNVRNA silencing suppressor p19 (19 kDa symptom severity modulator)
RDRP_CNVRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

4 entries

Cymbidium ringspot virus (CymRSV)

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CAPSD_CRVCapsid protein (Coat protein) (p41)
MVP_CRVMovement protein (p22)
P19_CRVRNA silencing suppressor p19 (19 kDa symptom severity modulator)
RDRP_CRVRNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

3 entries

Artichoke mottled crinkle virus (AMCV)

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CAPSD_AMCVCapsid protein (Coat protein) (p41)
MVP_AMCVMovement protein (p22)
P19_AMCVRNA silencing suppressor p19 (19 kDa symptom severity modulator)

3 entries

Tomato bushy stunt virus (strain BS-3) (TBSV)

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CAPSD_TBSVBCapsid protein (Coat protein) (p41)
MVP_TBSVBMovement protein (p22)
P19_TBSVBRNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain A23) (TBSV)

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MVP_TBSVAMovement protein (p22)
P19_TBSVARNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain B8) (TBSV)

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MVP_TBSV8Movement protein (p22)
P19_TBSV8RNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain Ja6) (TBSV)

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MVP_TBSVJMovement protein (p22)
P19_TBSVJRNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain Ja9) (TBSV)

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MP22_TBSVKMovement protein p22 (p21)
P19_TBSVKRNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain type) (TBSV)

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MP22_TBSVTMovement protein p22 (p21)
P19_TBSVTRNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Carnation Italian ringspot virus (CIRV)


P19_CIRVRNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Havel river virus (HaRV)


P19_HARVRNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Pear latent virus (PeLV)


P19_PLVRNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Pelargonium necrotic spot virus (PeNSV)


P19_PENSVRNA silencing suppressor p19 (19 kDa symptom severity modulator)