Nebovirus

VIRION

Non-enveloped, capsid of about 35 nm in diameter, with T=3 icosahedral symmetry. The capsid is composed of 180 capsid proteins. T=1 particles composed of 60 capsid proteins, about in diameter 15 nm are also observed. Genomic or subgenomic RNA are encapsidated in different particles.

GENOME

Monopartite, linear ssRNA(+) genome of 7.3 to 8.3 kb. The 5’-terminus is linked to a VPg protein and the 3’-terminus has a poly(A) tract.

GENE EXPRESSION

The virion RNA is infectious and serves as both genome and viral messenger RNA. Cleavage of ORF1 polyprotein by the virus-encoded 3C-like cysteine proteinase yields the non-structural proteins and the capsid protein. ORF2 encodes a small structural protein VP2 which is expressed through RNA termination-reinitiation.

REPLICATION

CYTOPLASMIC

  1. Attachement to host receptors mediates endocytosis of the virus into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. VPg is removed from the viral RNA, which is then translated into a processed ORF1 polyprotein to yield the replication proteins.
  4. Replication occurs in viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. Subgenomic RNA translation gives rise to the capsid protein and VP2.
  7. Assembly of new virus particles and release by cell lysis.

Matching UniProtKB/Swiss-Prot entries

4 entries grouped by strain (browse by keywords)

2 entries

Bovine enteric calicivirus Newbury agent-1 (isolate Bovine/UK/Newbury1/1976) (BEC) reference strain

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POLG_BECN1Genome polyprotein
VP2_BECN1Protein VP2 (Minor capsid VP2)

2 entries

Bovine enteric calicivirus NB (isolate Bovine/United States/Nebraska/1980) (BEC-NB)

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POLG_BECNBGenome polyprotein
VP2_BECNBProtein VP2 (Minor capsid protein)