Molecular biology

VIRION

Non-enveloped, with a diameter of about 30-32nm.

GENOME

ssDNA(+) genome of about 3.8 kb.
The genome is replicated through double-stranded intermediates. It may encode for 4 proteins: ORF1, ORF2, ORF3 and ORF4.

GENE EXPRESSION

REPLICATION

NUCLEAR

1. Virus penetrates into the host cell.
2. Uncoating, and release of the viral genomic ssDNA into the nucleus.
3. The ssDNA is converted into dsDNA with the participation of cellular factors.
4. viral mRNAs are transcribed and translated to produce viral proteins.
5. Replication may be mediated by a “Rep-like” protein, and would occur by rolling circle producing ssDNA genomes
6. These newly synthesized ssDNA can either
   a) be converted to dsDNA and serve as a template for transcription/replication
   b) be encapsidated by capsid protein and form virions released from the cell by budding