RNAse H are endonuclease that cleaves RNA strand in DNA-RNA duplex. This activity is coupled with reverse transcription to remove RNA template after it is retro-transcribed into DNA.


Enzymatic reaction:

  • RNA-DNA duplex dependent endoribonuclease EC

Mechanism Viral RNAse H are characterized by a conserved motif in which two conserved aspartic acid residues are bound to two magnesium ions. These ions interact with the phosphodiester bond. The reaction beginsby activation of a water molecule that acts as a nucleophile to attack the phosphate bond and eventually cleaves the phosphodiester bond, leaving a 3' hydroxyl and a 5' phosphate group.