Inoviridae

VIRION

image

Non-enveloped, rod of filaments of 7nm in diameter and 700 to 2000nm in length. Helical capsid with adsorption proteins on one end.

image

GENOME

Circular, ssDNA genome (+) of 4.5 to 8kb encoding for 4 to 10 proteins. Replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Each gene is transcribed by host cellular machinery, via a specific promoter. Some genes end by a transcription terminator.





REPLICATION

CYTOPLASMIC

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane.
  2. The proteins of the capsid perform the injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

replication cycle

image

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane.
  2. The proteins of the capsid mediate the Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

Matching UniProtKB/Swiss-Prot entries

87 entries grouped by strain (browse by keywords)

14 entries

Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

Select_allDeselect_all
VG430_BPPF1 Uncharacterized protein ORF430 (ORF430)
VG071_BPPF1 8.6 kDa protein (ORF 71)
VG424_BPPF1 Uncharacterized protein ORF424 (ORF 424)
VG090_BPPF1 10.1 kDa protein (ORF 90)
VG083_BPPF1 Uncharacterized protein ORF83 (ORF083)
VG030_BPPF1 3.2 kDa protein (ORF 30)
VG103_BPPF1 11.2 kDa protein (ORF 103)
CAPSD_BPPF1 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G3P_BPPF1 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
INTG_BPPF1 Putative integrase
G6P_BPPF1 Head virion protein G6P (Coat protein D) (G6P)
G5P_BPPF1 DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
VG110_BPPF1 12.0 kDa protein (ORF 110)
VG096_BPPF1 10.9 kDa protein (ORF 96)

9 entries

Enterobacteria phage IKe (Bacteriophage IKe) reference strain

Select_allDeselect_all
REP_BPIKE Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)
G4P_BPIKE Virion export protein (Gene 4 protein) (G4P)
G3P_BPIKE Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G9P_BPIKE Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G7P_BPIKE Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G5P_BPIKE DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
G6P_BPIKE Head virion protein G6P (Coat protein D) (G6P)
CAPSD_BPIKE Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G1P_BPIKE Gene 1 protein (G1P)

9 entries

Enterobacteria phage If1 (Bacteriophage If1) reference strain

Select_allDeselect_all
REP_BPIF1 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)
G4P_BPIF1 Virion export protein (Gene 4 protein) (G4P)
G5P_BPIF1 DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
G7P_BPIF1 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G9P_BPIF1 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
CAPSD_BPIF1 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G3P_BPIF1 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G6P_BPIF1 Head virion protein G6P (Coat protein D) (G6P)
G1P_BPIF1 Gene 1 protein (G1P)

9 entries

Enterobacteria phage M13 (Bacteriophage M13) reference strain

Select_allDeselect_all
REP_BPM13 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)
CAPSD_BPM13 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (M13 procoat) (Major coat protein)
G6P_BPM13 Head virion protein G6P (Coat protein D) (G6P)
G7P_BPM13 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G5P_BPM13 DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
G3P_BPM13 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G1P_BPM13 Gene 1 protein (G1P)
G4P_BPM13 Virion export protein (Gene 4 protein) (G4P)
G9P_BPM13 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)

9 entries

Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain

Select_allDeselect_all
REP_BPPF3 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)
VG430_BPPF3 Putative assembly protein ORF430 (ORF430)
VG301_BPPF3 Putative assembly protein ORF301 (ORF301)
G3P_BPPF3 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G5P_BPPF3 DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
G6P_BPPF3 Head virion protein G6P (Coat protein D) (G6P)
CAPSD_BPPF3 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein) (Pf3 coat protein)
VG058_BPPF3 6.4 kDa protein (ORF 58)
VG071_BPPF3 7.9 kDa protein (ORF 71)

8 entries

Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain

Select_allDeselect_all
REP_BPI22 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)
G9P_BPI22 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G3P_BPI22 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G1P_BPI22 Gene 1 protein (G1P)
G4P_BPI22 Virion export protein (Gene 4 protein) (G4P)
G5P_BPI22 DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
CAPSD_BPI22 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G7P_BPI22 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)

8 entries

Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain

Select_allDeselect_all
REP_BPPHL Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)
G7P_BPPHL Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
G3P_BPPHL Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G5P_BPPHL DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)
G9P_BPPHL Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
CAPSD_BPPHL Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G6P_BPPHL Head virion protein G6P (Coat protein D) (G6P)
G1P_BPPHL Gene 1 protein (G1P)

9 entries

Enterobacteria phage f1 (Bacteriophage f1)

Select_allDeselect_all
REP_BPF1 Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)
G4P_BPF1 Virion export protein (Gene 4 protein) (G4P)
G1P_BPF1 Gene 1 protein (G1P)
G9P_BPF1 Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G5P_BPF1 DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)
G3P_BPF1 Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G7P_BPF1 Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
CAPSD_BPF1 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G6P_BPF1 Head virion protein G6P (Coat protein D) (G6P)

9 entries

Enterobacteria phage fd (Bacteriophage fd)

Select_allDeselect_all
G1P_BPFD Gene 1 protein (G1P)
REP_BPFD Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)
G3P_BPFD Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)
G6P_BPFD Head virion protein G6P (Coat protein D) (G6P)
G9P_BPFD Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)
G7P_BPFD Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)
CAPSD_BPFD Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)
G4P_BPFD Virion export protein (Gene 4 protein) (G4P)
G5P_BPFD DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)

1 entry

Enterobacteria phage ZJ/2 (Bacteriophage ZJ-2)

CAPSD_BPZJ2 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)

1 entry

Thermus phage PH75 (Bacteriophage PH75)

CAPSD_BPH75 Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)

1 entry

Xanthomonas phage Xf (Bacteriophage Xf)

CAPSD_BPXF Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)