Lymphocryptovirus

VIRION

Enveloped, spherical to pleomorphic, 150-200 nm in diameter, T=16 icosahedral symmetry. Capsid consists of 162 capsomers and is surrounded by an amorphous tegument. Glycoproteins complexes are embedded in the lipid envelope.

GENOME

Monopartite, linear, dsDNA genome of about 180 kb. The genome contains terminal and internal reiterated sequences.

GENE EXPRESSION

Each viral transcript usually encodes a single protein and has a promoter/regulatory sequence, a TATA box, a transcription initiation site, a 5' leader sequence of 30-300 bp (not translated), a 3' untranslated sequence of 10-30 bp, and a poly A signal. There are many gene overlaps. There are only few spliced genes. Some of the expressed ORFs are antisense to each other. Some ORFs can be accessed from more than one promoter. There are some non-coding genes.

REPLICATION

NUCLEAR

Lytic replication:

  1. Attachment of the viral glycoproteins to host receptors mediates endocytosis of the virus into the host cell.
  2. Fusion with the plasma membrane to release the core and the tegument proteins into the host cytoplasm.
  3. The capsid is transported to the nuclear pore where the viral DNA is released into the nucleus.
  4. Transcription of immediate early genes which promote transcription of early genes and protect the virus against innate host immunity.
  5. Transcription of early viral mRNA by host polymerase II, encoding proteins involved in replication of the viral DNA.
  6. A first round of circular genome amplification occurs by bidirectional replication
  7. Synthesis of linear concatemer copies of viral DNA by rolling circle.
  8. Transcription of late mRNAs by host polymerase II, encoding structural proteins.
  9. Assembly of the virus in nuclear viral factories and budding through the inner lamella of the nuclear membrane which has been modified by the insertion of herpes glycoproteins, throughout the Golgi and final release at the plasma membrane.

Latent replication : replication of circular viral episome in tandem with the host cell DNA using the host cell replication machinery.

Host-virus interaction

Adaptive immune response inhibition

Epstein-Barr virus protein BNLF2a inhibits host adaptive immune response by interacting with TAP1 and TAP2 and thus preventing TAP-mediated peptide transport and subsequent loading. The glycine-alanine repeat domain (GAr) of Epstein-Barr virus-encoded nuclear antigen 1 (EBNA1) prevents major histocompatibility complex (MHC) class I-restricted presentation of EBNA1 epitopes to cytotoxic T cells.

Apoptosis modulation

Epstein-Barr viral protein BHRF1 is a viral homologue of the Bcl-2 oncogene that is able to protect cells from induced apoptosis. EBNA3C directly interacts with host Gemin3 and thereby blocks p53-mediated apoptosis .

Autophagy modulation

The viral Bcl-2 homolog BHRF1 in addition to prevent host cel death, may also control host autophagy .

Cell-cycle modulation

EBNA3C forms a complex with Cyclin D1 thereby facilitating the G1-S phase transition . Additionally, EBNA2 can affect activities of cell cycle regulators and retards cell cycle progression at G2/M phase. The conserved UL24 family of human alpha, beta and gamma herpesviruses induces a cell cycle arrest at G2/M transition through inactivation of the host cyclinB/cdc2 complex. EBV encodes an UL24 homolog termed BXRF1 that should perform this role .

Innate immune response inhibition

EBV inhibits the cascade leading to production of interferon-beta by targeting host IRF3 protein with the BGLF4 protein kinase that directly interacts with and inhibits host IRF3. BRLF1 expression also decreases induction of IFN-beta, and reduces expression of IRF3 and IRF7.

Host splicing inhibition

EBV EB2 modulates the host mRNA expression by exporting unspliced mRNA, thereby inducing alternative splicing .

Matching UniProtKB/Swiss-Prot entries

194 entries grouped by strain (browse by keywords)

All proteins are shown (view only 188 complete proteome entries)

76 entries

Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) reference strain

Select_allDeselect_all
DPOL_EBVB9 DNA polymerase catalytic subunit (EC 2.7.7.7)
EAR_EBVB9 Apoptosis regulator BHRF1 (Early antigen protein R) (EA-R) (Nuclear antigen)
EBNA4_EBVB9 Epstein-Barr nuclear antigen 4 (EBNA-4) (EBV nuclear antigen 4) (Epstein-Barr nuclear antigen 3B) ...
EBNA6_EBVB9 Epstein-Barr nuclear antigen 6 (EBNA-6) (EBV nuclear antigen 6) (Epstein-Barr nuclear antigen 3C) ...
EBNA5_EBVB9 Epstein-Barr nuclear antigen leader protein (EBNA-LP) (EBV nuclear antigen leader protein) ...
GM_EBVB9 Envelope glycoprotein M (gM)
GN_EBVB9 Envelope glycoprotein N
ICP27_EBVB9 mRNA export factor ICP27 homolog (Mta) (ORF57 protein homolog) (Protein SM)
MCP_EBVB9 Major capsid protein (MCP)
LTP_EBVB9 Large tegument protein deneddylase (EC 3.4.19.12) (EC 3.4.22.-)
LMP2_EBVB9 Latent membrane protein 2 (Terminal protein)
KITH_EBVB9 Thymidine kinase (EC 2.7.1.21)
LF1_EBVB9 Uncharacterized LF1 protein
LF2_EBVB9 Protein LF2
LF3_EBVB9 Uncharacterized protein LF3
KR2_EBVB9 Serine/threonine-protein kinase BGLF4 (EC 2.7.11.1)
ITP_EBVB9 Inner tegument protein
NEC1_EBVB9 Nuclear egress protein 1
MTP_EBVB9 Major tegument protein (MTP) (Protein p140)
LMP1_EBVB9 Latent membrane protein 1 (LMP-1) (Protein p63) [Cleaved into: Protein p25]
NEC2_EBVB9 Nuclear egress protein 2
GL_EBVB9 Envelope glycoprotein L (gL)
GP42_EBVB9 Glycoprotein 42 (gp42) [Cleaved into: Soluble gp42]
EAD_EBVB9 DNA polymerase processivity factor BMRF1 (Early antigen protein D) (EA-D) (Polymerase accessory ...
GH_EBVB9 Envelope glycoprotein H (gH)
GP350_EBVB9 Envelope glycoprotein GP350 (Membrane antigen) (MA)
EBNA1_EBVB9 Epstein-Barr nuclear antigen 1 (EBNA-1) (EBV nuclear antigen 1)
EBNA3_EBVB9 Epstein-Barr nuclear antigen 3 (EBNA-3) (EBV nuclear antigen 3) (Epstein-Barr nuclear antigen 3A) ...
DNBI_EBVB9 Major DNA-binding protein
DUT_EBVB9 Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (EC 3.6.1.23) (dUTP pyrophosphatase)
EBNA2_EBVB9 Epstein-Barr nuclear antigen 2 (EBNA-2) (EBV nuclear antigen 2)
IL10H_EBVB9 Viral interleukin-10 homolog (vIL-10) (20 kDa protein) (Protein BCRF1)
PRIM_EBVB9 DNA primase (EC 2.7.7.-)
RIR2_EBVB9 Ribonucleoside-diphosphate reductase small subunit (EC 1.17.4.1) (Ribonucleotide reductase small ...
PORTL_EBVB9 Portal protein
RIR1_EBVB9 Ribonucleoside-diphosphate reductase large subunit (R1) (EC 1.17.4.1) (Ribonucleotide reductase ...
RPMS1_EBVB9 Uncharacterized protein RPMS1
TRX1_EBVB9 Triplex capsid protein 1
GB_EBVB9 Envelope glycoprotein B (gB)
RTA_EBVB9 Replication and transcription activator (Rta) (Immediate-early protein Rta)
TEG7_EBVB9 Tegument protein UL51 homolog
TRM1_EBVB9 Tripartite terminase subunit 1
SCP_EBVB9 Small capsomere-interacting protein
SCAF_EBVB9 Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR) (Protein EC-RF3/EC-RF3A) ...
TRM3_EBVB9 Tripartite terminase subunit 3 (EC 3.1.-.-) (Terminase large subunit)
TRX2_EBVB9 Triplex capsid protein 2
BALF1_EBVB9 Apoptosis regulator BALF1
BZLF1_EBVB9 Trans-activator protein BZLF1 (EB1) (Zebra)
BRRF2_EBVB9 Tegument protein BRRF2
BTRF1_EBVB9 Uncharacterized protein BTRF1
BKRF4_EBVB9 Tegument protein BKRF4
BRRF1_EBVB9 Transcriptional activator BRRF1
BLRF2_EBVB9 Tegument protein BLRF2
BDLF2_EBVB9 Protein BDLF2
BHLF1_EBVB9 Uncharacterized protein BHLF1
BILF1_EBVB9 G-protein coupled receptor BILF1
BLLF2_EBVB9 Uncharacterized protein BLLF2
BNL2A_EBVB9 Protein BNLF2a
BNL2B_EBVB9 Uncharacterized protein BNLF2b
BMRF2_EBVB9 Protein BMRF2
BARF1_EBVB9 Secreted protein BARF1 (33 kDa early protein) (p33)
BDLF3_EBVB9 Glycoprotein BDLF3
BILF2_EBVB9 Glycoprotein BILF2
CVC2_EBVB9 Capsid vertex component 2
BFRF2_EBVB9 Uncharacterized protein BFRF2
CVC1_EBVB9 Capsid vertex component 1
CEP1_EBVB9 Cytoplasmic envelopment protein 1
CEP2_EBVB9 Cytoplasmic envelopment protein 2
CEP3_EBVB9 Cytoplasmic envelopment protein 3
AN_EBVB9 Shutoff alkaline exonuclease (SOX) (EC 3.1.-.-)
UNG_EBVB9 Uracil-DNA glycosylase (UDG) (EC 3.2.2.27) (UNG)
UL24_EBVB9 Protein UL24 homolog
UL32_EBVB9 Packaging protein UL32 homolog
UL87_EBVB9 TBP-like protein BcRF1
UL92_EBVB9 Uncharacterized protein BDLF4
UL95_EBVB9 Protein BGLF3

56 entries

Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4) reference strain

Select_allDeselect_all
DPOL_EBVA8 DNA polymerase catalytic subunit (EC 2.7.7.7)
EAR_EBVA8 Apoptosis regulator BHRF1 (Early antigen protein R) (EA-R) (Nuclear antigen)
EBNA5_EBVA8 Epstein-Barr nuclear antigen leader protein (EBNA-LP) (EBV nuclear antigen leader protein) ...
EBNA6_EBVA8 Epstein-Barr nuclear antigen 6 (EBNA-6) (EBV nuclear antigen 6) (Epstein-Barr nuclear antigen 3C) ...
EAD_EBVA8 DNA polymerase processivity factor BMRF1 (Early antigen protein D) (EA-D) (Polymerase accessory ...
EBNA4_EBVA8 Epstein-Barr nuclear antigen 4 (EBNA-4) (EBV nuclear antigen 4) (Epstein-Barr nuclear antigen 3B) ...
DUT_EBVA8 Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (EC 3.6.1.23) (dUTP pyrophosphatase)
EBNA1_EBVA8 Epstein-Barr nuclear antigen 1 (EBNA-1) (EBV nuclear antigen 1)
GM_EBVA8 Envelope glycoprotein M (gM)
MCP_EBVA8 Major capsid protein (MCP)
ICP27_EBVA8 mRNA export factor ICP27 homolog (Mta) (ORF57 protein homolog) (Protein SM)
LMP1_EBVA8 Latent membrane protein 1 (LMP-1) (Protein p63)
LMP2_EBVA8 Latent membrane protein 2 (Terminal protein)
LTP_EBVA8 Large tegument protein deneddylase (EC 3.4.19.12) (EC 3.4.22.-)
ITP_EBVA8 Inner tegument protein
LF1_EBVA8 Uncharacterized LF1 protein
KR2_EBVA8 Serine/threonine-protein kinase BGLF4 (EC 2.7.11.1)
LF2_EBVA8 Protein LF2
KITH_EBVA8 Thymidine kinase (EC 2.7.1.21)
NEC1_EBVA8 Nuclear egress protein 1
MTP_EBVA8 Major tegument protein (MTP) (Protein p140)
GL_EBVA8 Envelope glycoprotein L (gL)
GH_EBVA8 Envelope glycoprotein H (gH)
GN_EBVA8 Envelope glycoprotein N
GP42_EBVA8 Glycoprotein 42 (gp42)
GP350_EBVA8 Envelope glycoprotein GP350 (Membrane antigen) (MA)
DNBI_EBVA8 Major DNA-binding protein
GB_EBVA8 Envelope glycoprotein B (gB)
EBNA2_EBVA8 Epstein-Barr nuclear antigen 2 (EBNA-2) (EBV nuclear antigen 2)
EBNA3_EBVA8 Epstein-Barr nuclear antigen 3 (EBNA-3) (EBV nuclear antigen 3) (Epstein-Barr nuclear antigen 3A) ...
IL10H_EBVA8 Viral interleukin-10 homolog (vIL-10) (20 kDa protein) (Protein BCRF1)
PORTL_EBVA8 Portal protein
RIR1_EBVA8 Ribonucleoside-diphosphate reductase large subunit (R1) (EC 1.17.4.1) (Ribonucleotide reductase ...
RTA_EBVA8 Replication and transcription activator (Rta) (Immediate-early protein Rta)
TEG7_EBVA8 Tegument protein UL51 homolog
SCAF_EBVA8 Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR) (Protein EC-RF3/EC-RF3A) ...
TRM3_EBVA8 Tripartite terminase subunit 3 (EC 3.1.-.-) (Terminase large subunit)
RIR2_EBVA8 Ribonucleoside-diphosphate reductase small subunit (EC 1.17.4.1) (Ribonucleotide reductase small ...
CVC2_EBVA8 Capsid vertex component 2
BMRF2_EBVA8 Protein BMRF2
BNL2A_EBVA8 Protein BNLF2a
BRRF2_EBVA8 Tegument protein BRRF2
BKRF4_EBVA8 Tegument protein BKRF4
BRRF1_EBVA8 Transcriptional activator BRRF1
BLLF2_EBVA8 Uncharacterized protein BLLF2
BNL2B_EBVA8 Uncharacterized protein BNLF2b
BLRF2_EBVA8 Tegument protein BLRF2
BDLF2_EBVA8 Protein BDLF2
BARF1_EBVA8 Secreted protein BARF1 (33 kDa early protein) (p33)
BTRF1_EBVA8 Uncharacterized protein BTRF1
BZLF1_EBVA8 Trans-activator protein BZLF1 (EB1) (Zebra)
CEP2_EBVA8 Cytoplasmic envelopment protein 2
CEP1_EBVA8 Cytoplasmic envelopment protein 1
CEP3_EBVA8 Cytoplasmic envelopment protein 3
AN_EBVA8 Shutoff alkaline exonuclease (SOX) (EC 3.1.-.-)
UL92_EBVA8 Uncharacterized protein BDLF4

3 entries

Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)

Select_allDeselect_all
HELI_EBVB9 DNA replication helicase (EC 3.6.4.-)
YBL2_EBVB9 Uncharacterized protein BBLF2
YEC4_EBVB9 Uncharacterized protein EC-RF4

56 entries

Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)

Select_allDeselect_all
EBNA6_EBVG Epstein-Barr nuclear antigen 6 (EBNA-6) (EBV nuclear antigen 6) (Epstein-Barr nuclear antigen 3C) ...
EAD_EBVG DNA polymerase processivity factor BMRF1 (Early antigen protein D) (EA-D) (Polymerase accessory ...
EBNA5_EBVG Epstein-Barr nuclear antigen leader protein (EBNA-LP) (EBV nuclear antigen leader protein) ...
EBNA2_EBVG Epstein-Barr nuclear antigen 2 (EBNA-2) (EBV nuclear antigen 2)
EAR_EBVG Apoptosis regulator BHRF1 (Early antigen protein R) (EA-R) (Nuclear antigen)
EBNA3_EBVG Epstein-Barr nuclear antigen 3 (EBNA-3) (EBV nuclear antigen 3) (Epstein-Barr nuclear antigen 3A) ...
ICP27_EBVG mRNA export factor ICP27 homolog (Mta) (ORF57 protein homolog) (Protein SM)
MCP_EBVG Major capsid protein (MCP)
LTP_EBVG Large tegument protein deneddylase (EC 3.4.19.12) (EC 3.4.22.-)
LMP1_EBVG Latent membrane protein 1 (LMP-1) (Protein p63)
LMP2_EBVG Latent membrane protein 2 (Terminal protein)
KITH_EBVG Thymidine kinase (EC 2.7.1.21)
KR2_EBVG Serine/threonine-protein kinase BGLF4 (EC 2.7.11.1)
LF1_EBVG Uncharacterized LF1 protein
LF2_EBVG Protein LF2
LF3_EBVG Uncharacterized protein LF3
IL10H_EBVG Viral interleukin-10 homolog (vIL-10) (20 kDa protein) (Protein BCRF1)
MTP_EBVG Major tegument protein (MTP) (Protein p140)
DNBI_EBVG Major DNA-binding protein
GN_EBVG Envelope glycoprotein N
GL_EBVG Envelope glycoprotein L (gL)
GM_EBVG Envelope glycoprotein M (gM)
GP42_EBVG Glycoprotein 42 (gp42) [Cleaved into: Soluble gp42]
GH_EBVG Envelope glycoprotein H (gH)
GP350_EBVG Envelope glycoprotein GP350 (Membrane antigen) (MA)
DPOL_EBVG DNA polymerase catalytic subunit (EC 2.7.7.7)
DUT_EBVG Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (EC 3.6.1.23) (dUTP pyrophosphatase)
EBNA1_EBVG Epstein-Barr nuclear antigen 1 (EBNA-1) (EBV nuclear antigen 1)
EBNA4_EBVG Epstein-Barr nuclear antigen 4 (EBNA-4) (EBV nuclear antigen 4) (Epstein-Barr nuclear antigen 3B) ...
PORTL_EBVG Portal protein
RIR1_EBVG Ribonucleoside-diphosphate reductase large subunit (R1) (EC 1.17.4.1) (Ribonucleotide reductase ...
RIR2_EBVG Ribonucleoside-diphosphate reductase small subunit (EC 1.17.4.1) (Ribonucleotide reductase small ...
RPMS1_EBVG Uncharacterized protein RPMS1
TRM3_EBVG Tripartite terminase subunit 3 (EC 3.1.-.-) (Terminase large subunit)
SCAF_EBVG Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR) (Protein EC-RF3/EC-RF3A) ...
GB_EBVG Envelope glycoprotein B (gB)
ITP_EBVG Inner tegument protein
TEG7_EBVG Tegument protein UL51 homolog
RTA_EBVG Replication and transcription activator (Rta) (Immediate-early protein Rta)
BDLF2_EBVG Protein BDLF2
BMRF2_EBVG Protein BMRF2
BRRF2_EBVG Tegument protein BRRF2
BTRF1_EBVG Uncharacterized protein BTRF1
BARF1_EBVG Secreted protein BARF1 (33 kDa early protein) (p33)
BRRF1_EBVG Transcriptional activator BRRF1
BKRF4_EBVG Tegument protein BKRF4
BNL2B_EBVG Uncharacterized protein BNLF2b
BLRF2_EBVG Tegument protein BLRF2
BZLF1_EBVG Trans-activator protein BZLF1 (EB1) (Zebra)
BLLF2_EBVG Uncharacterized protein BLLF2
BNL2A_EBVG Protein BNLF2a
CVC2_EBVG Capsid vertex component 2
BALF1_EBVG Apoptosis regulator BALF1
CEP2_EBVG Cytoplasmic envelopment protein 2
CEP1_EBVG Cytoplasmic envelopment protein 1
AN_EBVG Shutoff alkaline exonuclease (SOX) (EC 3.1.-.-)

1 entry

Epstein-Barr virus (strain Cao) (HHV-4) (Human herpesvirus 4)

LMP1_EBVC Latent membrane protein 1 (LMP-1) (Protein p63)

1 entry

Epstein-Barr virus (strain P3HR-1) (HHV-4) (Human herpesvirus 4)

VGP3_EBVP3 Envelope glycoprotein GP340 (Membrane antigen) (MA)

1 entry

Epstein-Barr virus (strain Raji) (HHV-4) (Human herpesvirus 4)

LMP1_EBVR Latent membrane protein 1 (LMP-1) (Protein p63)