Cytoplasmic capsid assembly/packaging
Capsid assembly: refers to the formation of the capsid shell.
Packaging: refers to the viral genome placement inside a capsid or an envelope.
Helical capsids are assembled around genomic RNA or DNA, and rely on self and nucleic acid interactions to assemble. Assembly and packaging are linked. -<u>Negatives stranded RNA viruses genome is concomitantly encapsidated during replication. The packaging of these viruses occurs prior budding at the plasma membrane . Icosahedric capsids usually assemble by affinity around the viral genome. Complex capsids need the help of scaffolding proteins to assemble into empty procapsids . The scaffolding proteins are removed from the empty capsid by maturation events before packaging. -<u>Nucleo-Cytoplasmic Large DNA viruses (NCLDV) contain an internal membrane, consequently they have a complex and regulated assembly mechanism. Poxviridae capsid-like protein is removed before virion maturation and serves as a scaffolding protein . -<u>"Reoviridae":/by_species/104 and Totiviridae have their capsid assembled around messenger RNAs that are later replicated into genomic dsRNA, thereby hiding the dsRNA from cellular antiviral sensors.