Non-enveloped virion, about 40 nm in diameter with a T=4 icosahedral symmetry (240 copies of two proteins derived from the capsid precursor cleavage). The capsid precursor is expressed from a subgenomic RNA molecule which, along with genomic RNA, can be packaged into virions.
Linear ssRNA(+) genome of 6.1 kb without a polyA tail. There are three ORFs. The replicase ORF encodes two proteins via a suppression of termination mechanism.
The virion RNA is infectious and serves as both the genome and viral messenger RNA. Genomic RNA is translated into a polyprotein which is cleaved presumably by a viral protease into replication proteins and a 130 kDa protein. Replication produces a subgenomic RNA which encodes for capsid protein.
- Virus penetrates into the host cell.
- Uncoating, and release of the viral genomic RNA into the cytoplasm.
- Genomic RNA is translated to produce replication proteins.
- Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
- The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
- Expression of the subgenomic RNA encoding the capsid protein.
- Assembly of new virus particles.
- Virion maturation by the viral protease.