Non-enveloped, head-tail structure. The prolate head is about 145 nm x 44 nm and the tail 13 nm x 8 nm, with short, possibly kinked tail fibers folded on the tail.


Linear, dsDNA genome of about 77 kb encoding for 128 genes.




  1. Adsorption: the phage attaches to the target cell through its tail fibers.
  2. Ejection of the viral DNA into host cell cytoplasm.
  3. Transcription and translation of early genes.
  4. Replication of genomic DNA. Transcription and translation of late genes.
  5. Assembly of an empty procapsid and packaging of the genome.
  6. Viral tail fibers assembly and viral tail assembly.
  7. Mature virions are released from the cell by lysis.

Matching UniProtKB/Swiss-Prot entries

5 entries grouped by strain (browse by keywords)

5 entries

Escherichia phage Phieco32 (Escherichia coli phage phi32) reference strain

CAPSD_BPE32 Major capsid protein (Gene product 11) (gp11) (Major head protein)
FIB20_BPE32 Putative tail tip fiber protein (Gene product 20) (gp20)
FIB11_BPE32 Probable tail fiber protein (Gene product 11) (gp11)
EXO74_BPE32 3'-5' exonuclease gp74 (EC 3.1.11.-)
DPOL_BPE32 DNA-directed DNA polymerase (EC (EC 3.1.11.-) (DNA polymerase gp53)