VIRION
GENOME
Linear, dsDNA genome of about 10 kb with inverted terminal repeats and terminal proteins covalently linked to it.
GENE EXPRESSION
The genome encodes for about 21 proteins.
ENZYMES
REPLICATION
CYTOPLASMIC
- Adsorption: the phage attach to target cell
- Injection: viral DNA is injected in host cell cytoplasm
- Transcription and translation of early genes
- Replication of genomic DNA via a rolling-circle mechanism
- Transcription and translation of late genes
- Capsid proteins polymerize around a lipoprotein vesicle translocated in the cytoplasm by virion assembly factors.
- Genomic DNA is packaged in new virions
- Mature virions are released from the cell by lysis