VIRION
Presumably icosahedral virion, 80nm in diameter.
- Double jelly roll-fold major capsid protein [MCP]
GENOME
Linear, dsDNA genome of about 45kb kb.
GENE EXPRESSION
The genome encodes for about 74 proteins.
ENZYMES
REPLICATION
CYTOPLASMIC
- Adsorption: the phage attach to target cell
- Injection: viral DNA is injected in host cell cytoplasm
- Transcription and translation of early genes
- Replication of genomic DNA via a rolling-circle mechanism
- Transcription and translation of late genes
- Capsid proteins polymerize around a lipoprotein vesicle translocated in the cytoplasm by virion assembly factors.
- Genomic DNA is packaged in new virions
- Mature virions are released from the cell by lysis