Kusarnavirus (taxid:2560164)

VIRION

Presumably non-enveloped, pseudo T=3 icosahedral capsid, about 25nm in diameter and composed of 60 trimers of VP1-VP2-VP3 proteins.

GENOME

Monopartite, linear ssRNA(+) genome about 8.8 kb in size.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Encodes for two open reading frames.

ENZYMES

• RNA-dependent RNA polymerase [RdRp A0A077JBM9]
• VPG-type capping [VPg A0A077JBM9]
• NTPase-helicase [NTPase A0A077JBM9]
• Polyprotein major protease (Peptidase C3) [Protease A0A077JBM9]

REPLICATION

1. Virus penetrates into the host cell.
2. Uncoating, and release of the viral genomic RNA into the cytoplasm possibly through the formation of a pore in the host cell membrane.
3. Synthesis and proteolytic cleavage of the polyprotein.
4. Replication occurs in viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
6. Assembly of new virions.
7. Virus release.