Wumivirus (taxid:2509285)

VIRION

Unknown, discovered using viral metagenomics. Presumably enveloped.

GENOME

Segmented Negative-stranded RNA linear genome, L segment is about 12 kb, M segment about 7 kb and S segment 2 kb. Encodes at least three proteins.

Enzymes: RNA dependent RNA polymerase, endonuclease? (Cap snatching).

GENE EXPRESSION

The viral RNA dependent RNA polymerase (L) binds to a promoter on each encapsidated segment, and transcribes the mRNA. These are capped by L protein during synthesis using cap snatching .

ENZYMES

• RNA-dependent RNA polymerase [L]
• Cap Snatching endonuclease [L]

REPLICATION

CYTOPLASMIC

1. Virus attaches to host receptors though glycoprotein, and is endocytosed into vesicles in the host cell.
2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid segments are released in the cytoplasm.
3. Transcription, viral mRNAs are capped in the cytoplasm.
4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
5. The ribonucleocapsids buds, presumably releasing the virion by exocytosis.