Molecular biology

VIRION

Enveloped, spherical. The virions are 80-120 nm in diameter

GENOME

segmented ssRNA(-) linear genome, encapsidated by nucleoprotein (NP) Contains 6 to 7 segments coding for 7 to 9 proteins. Segments size range from 0.9 to 2.3 kb. Genome total size is about 10 Kb

GENE EXPRESSION

Viral RNA polymerase (PB1, PB2 and PA) transcribes one mRNA from each genome segment. Transcription is primed by cap cleaved from cellular mRNAS by Cap snatching. mRNA are polyadenylated by the viral polymerase stuttering on a poly U track. Splicing of segment 6 produces a mRNA encoding for M1 protein.

REPLICATION

NUCLEUS

1. Virus attaches to sialic acid receptor though GP protein and is endocytosed in the host cell.
2. endosome acidification induces fusion of virus membrane with the vesicle membrane; encapsidated RNA segments migrate to the nucleus.
3. Transcription of genomic segments by the viral polymerase produces mRNAs.
4. genomic (-)RNA is replicated through antigenomic (+)RNA intermediate.
5. High level of M1 may protein induces genomes segments export from nucleus.
6. Virus assembly and budding occurs at the plasma membrane.