Ravinvirus

VIRION

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Non-enveloped, head-tail structure. The head is about 60 nm in diameter. The tail is flexible, about 140 nm in length and 8 nm in width, with short brush-like terminal fibers.

GENOME

Linear, dsDNA genome of about 46 kb with covalently closed hairpin ends, encoding about 60 proteins. The genome has cohesive ends (cos sites) .

GENE EXPRESSION

REPLICATION

CYTOPLASMIC

  1. Adsorption: The phage attaches to target cell adhesion receptors through its tail fibers.
  2. Ejection of the viral DNA into host cell cytoplasm by long flexible tail ejection system.
  3. Genome circularization
  4. Protelomerase cuts at another site, giving rise to a linear plasmid
  5. Transcription and translation of early genes.
  6. Replication of genomic DNA by dsDNA bidirectional replication.
  7. Transcription and translation of late genes
  8. Assembly of an empty procapsid and packaging of the genome.
  9. Viral tail fiber assembly and viral tail assembly.
  10. Mature virions are released from the cell by lysis.