Tombusviridae

VIRION

Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).

GENOME

Monopartite (except Dianthovirus which is bipartite), linear, ssRNA(+) genome of 4-5.4 kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. The downstream genes encoding the movement protein, and capsid proteins are translated from subgenomic RNAs. viral suppressor of RNA silencing (VSRs) is expressed by leaky scanning of the MVP subgenomic mRNA.
Cap-independent translation is mediated possibly through the interaction between a 3'-cap-independent translation element and a 5’UTR stem-loop.

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3’co-terminal subgenomic RNAs that will generate the capsid and movement protein.
  7. Virus assembly in the cytoplasm.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

Viruses of the Tombusviridae family encode proteins acting as suppressors of RNA silencing. Examples are tombusvirus P19/P20 , aureusvirus P14 , dianthovirus movement protein , carmovirus capsid protein P38 .

Matching UniProtKB/Swiss-Prot entries

80 entries grouped by strain (browse by keywords)

All proteins are shown (view only 58 complete proteome entries)

5 entries

Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

Select_allDeselect_all
RDRP_PMVK RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p112) [Cleaved into: Protein p48]
P15_PMVK Protein p15
MP6_PMVK Putative movement protein p6.6
MP8_PMVK Probable movement protein p8
CAPSD_PMVK Capsid protein (Coat protein) (p26)

5 entries

Tobacco necrosis virus (strain A) (TNV-A) reference strain

Select_allDeselect_all
RDRP_TNVA RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82) [Cleaved into: Protein p23]
P7_TNVA Uncharacterized protein p7
MP8_TNVA Probable movement protein p8
P6_TNVA Uncharacterized protein p6
CAPSD_TNVA Capsid protein (p30)

4 entries

Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain

Select_allDeselect_all
RDRP_CARMS Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88) [Cleaved into: Protein p28]
MP1_CARMS Double gene block protein 1 (DGBp1) (Movement protein P7)
MP2_CARMS Double gene block protein 2 (DGBp2) (Movement protein P9)
CAPSD_CARMS Capsid protein (Coat protein) (p38)

4 entries

Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

Select_allDeselect_all
RDRP_MCMVK RNA-directed RNA polymerase (EC 2.7.7.48) (111 kDa protein) (Protein p111) [Cleaved into: Protein ...
P31_MCMVK Uncharacterized protein p31 [Cleaved into: Uncharacterized protein p7]
P32_MCMVK Uncharacterized protein p32
CAPSD_MCMVK Capsid protein (Coat protein) (p25)

4 entries

Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

Select_allDeselect_all
RDRP_POLVP RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84) [Cleaved into: Protein p25]
MVP_POLVP Movement protein (p27)
P14_POLVP RNA silencing suppressor p14
CAPSD_POLVP Capsid protein (p40)

4 entries

Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain

Select_allDeselect_all
RDRP_TBSVC RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92) [Cleaved into: Protein p33]
MVP_TBSVC Movement protein (p22)
P19_TBSVC RNA silencing suppressor p19 (19 kDa symptom severity modulator)
CAPSD_TBSVC Capsid protein (Coat protein) (p41)

4 entries

Turnip crinkle virus (TCV) reference strain

Select_allDeselect_all
RDRP_TCV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88) [Cleaved into: Protein p28]
MP1_TCV Double gene block protein 1 (DGBp1) (Movement protein P8)
MP2_TCV Double gene block protein 2 (DGBp2) (Movement protein P9)
CAPSD_TCV Capsid protein (Coat protein) (p38)

3 entries

Carnation ringspot virus (isolate Lommel) (CRSV) reference strain

Select_allDeselect_all
RDRP_CRSVL RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)
MP_CRSVL Movement protein (MP)
CAPSD_CRSVL Capsid protein (Coat protein)

3 entries

Groundnut rosette virus (strain MC1) (GRV) reference strain

Select_allDeselect_all
RDRP_GRVMC RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48)
ORF3_GRVMC Long-distance movement protein (ORF3 protein)
MVP_GRVMC Movement protein

3 entries

Melon necrotic spot virus (MNSV) reference strain

Select_allDeselect_all
MP1_MNSV Double gene block protein 1 (DGBp1) (Movement protein P7A)
MP2_MNSV Double gene block protein 2 (DGBp2) (Movement protein P7B)
CAPSD_MNSV Capsid protein (Coat protein) (p42)

3 entries

Oat chlorotic stunt virus (isolate United Kingdom) (OCSV) reference strain

Select_allDeselect_all
RDRP_OCSVU RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84) [Cleaved into: Protein p23]
P8_OCSVU Uncharacterized protein p8
CAPSD_OCSVU Capsid protein (p48)

3 entries

Red clover necrotic mosaic virus (RCNMV) reference strain

Select_allDeselect_all
RDRP_RCNMV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)
MP_RCNMV Movement protein (MP)
CAPSD_RCNMV Capsid protein (Coat protein)

4 entries

Cucumber necrosis virus (CNV)

Select_allDeselect_all
RDRP_CNV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92) [Cleaved into: Protein p33]
P19_CNV RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MVP_CNV Movement protein (p22)
CAPSD_CNV Capsid protein (Coat protein) (p41)

4 entries

Cymbidium ringspot virus (CymRSV)

Select_allDeselect_all
RDRP_CRV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92) [Cleaved into: Protein p33]
MVP_CRV Movement protein (p22)
P19_CRV RNA silencing suppressor p19 (19 kDa symptom severity modulator)
CAPSD_CRV Capsid protein (Coat protein) (p41)

4 entries

Tobacco necrosis virus (strain D) (TNV-D)

Select_allDeselect_all
RDRP_TNVD RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82) [Cleaved into: Protein p23]
P6_TNVD Uncharacterized protein p6
MP8_TNVD Probable movement protein p8
CAPSD_TNVD Capsid protein (p30)

3 entries

Artichoke mottled crinkle virus (AMCV)

Select_allDeselect_all
MVP_AMCV Movement protein (p22)
P19_AMCV RNA silencing suppressor p19 (19 kDa symptom severity modulator)
CAPSD_AMCV Capsid protein (Coat protein) (p41)

3 entries

Carnation mottle virus (CarMV)

Select_allDeselect_all
RDRP_CARMV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88) [Cleaved into: Protein p28]
MP1_CARMV Double gene block protein 1 (DGBp1) (Movement protein P7)
CAPSD_CARMV Capsid protein (Coat protein) (p38)

3 entries

Tomato bushy stunt virus (strain BS-3) (TBSV)

Select_allDeselect_all
MVP_TBSVB Movement protein (p22)
P19_TBSVB RNA silencing suppressor p19 (19 kDa symptom severity modulator)
CAPSD_TBSVB Capsid protein (Coat protein) (p41)

2 entries

Tomato bushy stunt virus (strain A23) (TBSV)

Select_allDeselect_all
MVP_TBSVA Movement protein (p22)
P19_TBSVA RNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain B8) (TBSV)

Select_allDeselect_all
MVP_TBSV8 Movement protein (p22)
P19_TBSV8 RNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain Ja6) (TBSV)

Select_allDeselect_all
P19_TBSVJ RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MVP_TBSVJ Movement protein (p22)

2 entries

Tomato bushy stunt virus (strain Ja9) (TBSV)

Select_allDeselect_all
MP22_TBSVK Movement protein p22 (p21)
P19_TBSVK RNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain type) (TBSV)

Select_allDeselect_all
P19_TBSVT RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MP22_TBSVT Movement protein p22 (p21)

1 entry

Carnation Italian ringspot virus (CIRV)

P19_CIRV RNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Havel river virus (HaRV)

P19_HARV RNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Pear latent virus (PeLV)

P19_PLV RNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Pelargonium necrotic spot virus (PeNSV)

P19_PENSV RNA silencing suppressor p19 (19 kDa symptom severity modulator)