Locarnavirus (taxid:2560169)

VIRION

Presumably non-enveloped, pseudo T=3 icosahedral capsid, about 25nm in diameter and composed of 60 trimers of VP1-VP2-VP3 proteins.

GENOME

Monopartite, linear ssRNA(+) genome between 8.6 and 9.3 kb in size.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Encodes for two open reading frames.

ENZYMES

• RNA-dependent RNA polymerase [RdRp A7KCA2]
• VPG-type capping [VPg A7KCA2]
• NTPase-helicase [NTPase A7KCA2]
• Polyprotein major protease (Peptidase C3) [Protease A7KCA2]

REPLICATION

1. Virus penetrates into the host cell.
2. Uncoating, and release of the viral genomic RNA into the cytoplasm possibly through the formation of a pore in the host cell membrane.
3. Synthesis and proteolytic cleavage of the polyprotein.
4. Replication occurs in viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
6. Assembly of new virions.
7. Virus release.