Tobravirus (taxid:12293)

VIRION

Non-enveloped, rod shaped with helical symmetry. The virion is composed of two segments 180-215 and 46-115 nm in length, and 22 nm in diameter.

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GENOME

Segmented, linear, ssRNA(+) genome. The two segments are 6.8 and 4.5 kb in size respectively. The genomic RNAs are capped, not polyadenylated in 3'.

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GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. The RNA-1 5'proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. Subgenomic RNAs may be synthesized during replication of RNA-1 and RNA-2 and give rise to the movement protein and viral suppressor of RNA silencing.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3'co-terminal subgenomic RNAs that will generate the movement protein and viral suppressor of RNA silencing. The capsid protein is directly expressed from RNA-2.
  7. Virus assembly in the cytoplasm. Each segment (if multipartite) is encapsidated.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

The cysteine-rich protein of tobacco rattle virus act as suppressor of RNA silencing in roots, but not in leaves .

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

22 entries grouped by protein

1 entry

Replicase large subunit (EC 2.1.1.-) (EC 2.7.7.-) (EC 2.7.7.48) (EC 3.6.4.13) (194 kDa protein) (RNA-directed RNA polymerase)

194K_TRVSY
Tobacco rattle virus (strain SYM)

2 entries

Protein 2b (29.1 kDa protein)

Select_allDeselect_all
2B_PEBV
Pea early browning virus reference strain

Protein 2b (29.6 kDa protein)

2B_TRVTC
Tobacco rattle virus (strain TCM)

1 entry

Capsid protein (Coat protein) (P23)

CAPSD_TRVPP
Tobacco rattle virus (isolate PpK20) (TRV) reference strain

5 entries

Coat protein (Capsid protein)

Select_allDeselect_all
COAT_PEBV
Pea early browning virus reference strain
COAT_TRVCA
Tobacco rattle virus (strain CAM)
COAT_TRVPL
Tobacco rattle virus (strain PLB)
COAT_TRVPS
Tobacco rattle virus (strain PSG)
COAT_TRVTC
Tobacco rattle virus (strain TCM)

1 entry

Movement protein (29 kDa protein) (Cell-to-cell transport protein) (P1a)

MVP_TRVPP
Tobacco rattle virus (isolate PpK20) (TRV) reference strain

1 entry

32 kDa protein

P32_TRVPP
Tobacco rattle virus (isolate PpK20) (TRV) reference strain

1 entry

40 kDa protein

P40_TRVPP
Tobacco rattle virus (isolate PpK20) (TRV) reference strain

1 entry

Replicase large subunit (EC 2.1.1.-) (EC 2.7.7.-) (EC 2.7.7.48) (EC 3.6.4.13) (194 kDa protein) (RNA-directed RNA polymerase)

RDRP_TRVPP
Tobacco rattle virus (isolate PpK20) (TRV) reference strain

1 entry

16 kDa protein

V13K_TRVPL
Tobacco rattle virus (strain PLB)

4 entries

16 kDa protein

Select_allDeselect_all
V16K_TRVPL
Tobacco rattle virus (strain PLB)
V16K_TRVPS
Tobacco rattle virus (strain PSG)
V16K_TRVSY
Tobacco rattle virus (strain SYM)
V16K_TRVTC
Tobacco rattle virus (strain TCM)

1 entry

23 kDa protein

V23K_PEBV
Pea early browning virus reference strain

2 entries

29 kDa protein

Select_allDeselect_all
V29K_TRVPS
Tobacco rattle virus (strain PSG)
V29K_TRVSY
Tobacco rattle virus (strain SYM)

1 entry

Suppressor of RNA silencing (16kD cysteine rich protein) (P16)

VSR_TRVPP
Tobacco rattle virus (isolate PpK20) (TRV) reference strain