Tombusviridae (taxid:39738)

VIRION

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Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).
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GENOME

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Monopartite (except Dianthovirus which is bipartite), linear, ssRNA(+) genome of 4-5.4 kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1 (tombusvirus-like and carmovirus-like), or ribosomal frameshifting (Dianthovirus and umbravirus). The downstream genes encoding the movement protein, and capsid proteins are translated from subgenomic RNAs. viral suppressor of RNA silencing (VSRs) is expressed by leaky scanning of the MVP subgenomic mRNA.
Cap-independent translation is mediated possibly through the interaction between a 3'-cap-independent translation element and a 5'UTR stem-loop.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3'co-terminal subgenomic RNAs that will generate the capsid and movement protein.
  7. Virus assembly in the cytoplasm.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

Viruses of the Tombusviridae family encode proteins acting as suppressors of RNA silencing. Examples are tombusvirus P19/P20 , aureusvirus P14 , dianthovirus movement protein , carmovirus capsid protein P38 .

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

99 entries grouped by strain

6 entries

Barley yellow dwarf virus (isolate PAV) (BYDV) reference strain

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MCAPS_BYDVP Readthrough protein P3-RTD (P3-P5 readthrough protein) (P72) (Readthrough protein) (RT protein) ...
RDRP_BYDVP Putative RNA-directed RNA polymerase (EC 2.7.7.48)
CAPSD_BYDVP Major capsid protein (Coat protein) (CP)
MVP_BYDVP Movement protein (MP) (17 kDa protein)
ORF6_BYDVP Uncharacterized protein ORF6
ORF7_BYDVP Uncharacterized protein ORF7

5 entries

Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

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RDRP_MCMVK RNA-directed RNA polymerase (EC 2.7.7.48) (111 kDa protein) (Protein p111) [Cleaved into: Protein ...
MP2_MCMVK p7b
MP1_MCMVK Protein p31 [Cleaved into: Movement protein p7a]
CAPSD_MCMVK Capsid protein (Coat protein) (p25)
P32_MCMVK Uncharacterized protein p32

5 entries

Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

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RDRP_PMVK RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p112) [Cleaved into: Protein p48]
MP6_PMVK Putative movement protein p6.6
CAPSD_PMVK Capsid protein (Coat protein) (p26)
MP8_PMVK Probable movement protein p8
P15_PMVK Protein p15

5 entries

Tobacco necrosis virus (strain A) (TNV-A) reference strain

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CAPSD_TNVA Capsid protein (p30)
RDRP_TNVA RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82) [Cleaved into: Protein p23]
MP8_TNVA Probable movement protein p8
P6_TNVA Uncharacterized protein p6
P7_TNVA Uncharacterized protein p7

4 entries

Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain

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MP1_CARMS Double gene block protein 1 (DGBp1) (Movement protein P7)
MP2_CARMS Double gene block protein 2 (DGBp2) (Movement protein P9)
RDRP_CARMS Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88) [Cleaved into: Protein p28]
CAPSD_CARMS Capsid protein (Coat protein) (p38)

4 entries

Melon necrotic spot virus (MNSV) reference strain

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RDRP_MNSV RNA-directed RNA polymerase (EC 2.7.7.48) (p89) [Cleaved into: p29]
MP1_MNSV Double gene block protein 1 (DGBp1) (Movement protein P7A)
CAPSD_MNSV Capsid protein (Coat protein) (p42)
MP2_MNSV Double gene block protein 2 (DGBp2) (Movement protein P7B)

4 entries

Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

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RDRP_POLVP RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84) [Cleaved into: Protein p25]
MVP_POLVP Movement protein (p27)
CAPSD_POLVP Capsid protein (p40)
P14_POLVP RNA silencing suppressor p14

4 entries

Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain

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P19_TBSVC RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MVP_TBSVC Movement protein (p22)
CAPSD_TBSVC Capsid protein (Coat protein) (p41)
RDRP_TBSVC RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92) [Cleaved into: Protein p33]

4 entries

Turnip crinkle virus (TCV) reference strain

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CAPSD_TCV Capsid protein (Coat protein) (p38)
RDRP_TCV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88) [Cleaved into: Protein p28]
MP1_TCV Double gene block protein 1 (DGBp1) (Movement protein P8)
MP2_TCV Double gene block protein 2 (DGBp2) (Movement protein P9)

1 entry

Artichoke mottled crinkle virus (AMCV) reference strain

CAPSD_AMCV Capsid protein (Coat protein) (p41)

3 entries

Barley yellow dwarf virus (isolate MAV-PS1) (BYDV) reference strain

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RDRP_BYDV1 Putative RNA-directed RNA polymerase (EC 2.7.7.48)
CAPSD_BYDV1 Major capsid protein (Coat protein) (CP)
MVP_BYDV1 Movement protein (MP) (17 kDa protein)

3 entries

Carnation mottle virus (CarMV) reference strain

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CAPSD_CARMV Capsid protein (Coat protein) (p38)
RDRP_CARMV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88) [Cleaved into: Protein p28]
MP1_CARMV Double gene block protein 1 (DGBp1) (Movement protein P7)

3 entries

Groundnut rosette virus (strain MC1) (GRV) reference strain

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ORF3_GRVMC Long-distance movement protein (ORF3 protein)
RDRP_GRVMC RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48)
MVP_GRVMC Movement protein

3 entries

Oat chlorotic stunt virus (isolate United Kingdom) (OCSV) reference strain

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RDRP_OCSVU RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84) [Cleaved into: Protein p23]
CAPSD_OCSVU Capsid protein (p48)
P8_OCSVU Uncharacterized protein p8

3 entries

Red clover necrotic mosaic virus (RCNMV) reference strain

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RDRP_RCNMV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)
MP_RCNMV Movement protein (MP)
CAPSD_RCNMV Capsid protein (Coat protein)

4 entries

Cucumber necrosis virus (CNV)

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CAPSD_CNV Capsid protein (Coat protein) (p41)
P19_CNV RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MVP_CNV Movement protein (p22)
RDRP_CNV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92) [Cleaved into: Protein p33]

4 entries

Cymbidium ringspot virus (CymRSV)

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CAPSD_CRV Capsid protein (Coat protein) (p41)
P19_CRV RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MVP_CRV Movement protein (p22)
RDRP_CRV RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92) [Cleaved into: Protein p33]

4 entries

Tobacco necrosis virus (strain D) (TNV-D)

Select_allDeselect_all
RDRP_TNVD RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82) [Cleaved into: Protein p23]
CAPSD_TNVD Capsid protein (p30)
MP8_TNVD Probable movement protein p8
P6_TNVD Uncharacterized protein p6

2 entries

Artichoke mottled crinkle virus (AMCV)

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P19_AMCV RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MVP_AMCV Movement protein (p22)

3 entries

Barley yellow dwarf virus (isolate P-PAV) (BYDV)

Select_allDeselect_all
RDRP_BYDVR Putative RNA-directed RNA polymerase (EC 2.7.7.48)
MVP_BYDVR Movement protein (MP) (17 kDa protein)
CAPSD_BYDVR Major capsid protein (Coat protein) (CP)

3 entries

Carnation ringspot virus (isolate Lommel) (CRSV)

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RDRP_CRSVL RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)
CAPSD_CRSVL Capsid protein (Coat protein)
MP_CRSVL Movement protein (MP)

3 entries

Tomato bushy stunt virus (strain BS-3) (TBSV)

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CAPSD_TBSVB Capsid protein (Coat protein) (p41)
P19_TBSVB RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MVP_TBSVB Movement protein (p22)

2 entries

Barley yellow dwarf virus (isolate NY-RPV) (BYDV)

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CAPSD_BYDVN Major capsid protein (Coat protein) (CP)
MVP_BYDVN Movement protein (MP) (17 kDa protein)

2 entries

Bean leafroll virus (BLRV)

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CAPSD_BLRV Major capsid protein (Coat protein) (CP)
MVP_BLRV Movement protein (MP) (16 kDa protein)

2 entries

Tomato bushy stunt virus (strain A23) (TBSV)

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P19_TBSVA RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MVP_TBSVA Movement protein (p22)

2 entries

Tomato bushy stunt virus (strain B8) (TBSV)

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P19_TBSV8 RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MVP_TBSV8 Movement protein (p22)

2 entries

Tomato bushy stunt virus (strain Ja6) (TBSV)

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MVP_TBSVJ Movement protein (p22)
P19_TBSVJ RNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain Ja9) (TBSV)

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MP22_TBSVK Movement protein p22 (p21)
P19_TBSVK RNA silencing suppressor p19 (19 kDa symptom severity modulator)

2 entries

Tomato bushy stunt virus (strain type) (TBSV)

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P19_TBSVT RNA silencing suppressor p19 (19 kDa symptom severity modulator)
MP22_TBSVT Movement protein p22 (p21)

1 entry

Barley yellow dwarf virus (isolate MAV) (BYDV)

CAPSD_BYDVM Major capsid protein (Coat protein) (CP)

1 entry

Carnation Italian ringspot virus (CIRV)

P19_CIRV RNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Havel river virus (HaRV)

P19_HARV RNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Pear latent virus (PeLV)

P19_PLV RNA silencing suppressor p19 (19 kDa symptom severity modulator)

1 entry

Pelargonium necrotic spot virus (PeNSV)

P19_PENSV RNA silencing suppressor p19 (19 kDa symptom severity modulator)