Tospoviridae (taxid:1980419)

VIRION

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Enveloped, spherical. Diameter from 80 to 120nm.

GENOME

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Segmented Negative-stranded RNA linear genome, L segment is about 8.8kb, M segment about 4.8kb and S segment about 3kb.
Encodes for six proteins.

GENE EXPRESSION

Transcription starts by viral RNA dependent RNA polymerase (L) binding to a promoter on each encapsidated segment, and is terminated by a strong hairpin sequence at the end of each gene. mRNAs are capped by L protein during synthesis using cap snatching , but are not polyadenylated.
S and M segments uses ambisense strategy to encode forsevral proteins: both genomic ans antigenomic RNA are transcribed. The hairpin sequence is a stop polymerase signal which prevents ambisense transcription from producing dsRNA. MRNA1 encodes for a polyprotein which is cleaved by host protease into Gn and Gc proteins.

ENZYMES

REPLICATION

IN PLANT:CYTOPLASMIC

  1. Virus penetrates into the cell by effraction or plasmodesmata transport.
  2. Cytoplasmic transcription by viral polymerase on the RNA viral RNA template.
  3. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  4. The ribonucleocapsid can penetrate in neighboring plant cells through plasmodesmata transport
  5. Alternatively budding of particles occurs at the cell membrane, releasing new virions.

IN INSECT:CYTOPLASMIC

  1. Attachment of the viral G glycoproteins to host receptors.
  2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid is released into the cytoplasm.
  3. Cytoplasmic transcription by viral polymerase on the RNA viral RNA template.
  4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  5. Budding of particles occurs at the cell membrane, releasing new virions.

Host-virus interaction

Inhibition of host innate immunity

TSWV NSs inhibits Dicer-mediated dsRNA processing thereby inhibiting RNAi-mediated immunity to control innate immune signaling.

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

18 entries grouped by strain

5 entries

Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV) reference strain

Select_allDeselect_all
GP_TSWV1 Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G2); ...
L_TSWV1 RNA-directed RNA polymerase L (Protein L) (EC 2.7.7.48) (Large structural protein) (Replicase) ...
MVP_TSWV1 Movement protein (MVP) (Non-structural movement protein) (NSm)
NCAP_TSWV1 Nucleoprotein (Nucleocapsid protein) (Protein N)
NSS_TSWV1 Non-structural protein NS-S

3 entries

Impatiens necrotic spot virus (INSV) reference strain

Select_allDeselect_all
GP_INSV Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G1); ...
MVP_INSV Movement protein (MVP) (Non-structural movement protein) (NSm)
NCAP_INSV Nucleoprotein (Nucleocapsid protein) (Protein N)

2 entries

Impatiens necrotic spot virus (strain NL-07) (INSV)

Select_allDeselect_all
NCAP_INSVN Nucleoprotein (Nucleocapsid protein) (Protein N)
NSS_INSVN Non-structural protein NS-S

2 entries

Tomato spotted wilt virus (strain Bulgarian L3) (TSWV)

Select_allDeselect_all
NCAP_TSWVL Nucleoprotein (Nucleocapsid protein) (Protein N)
NSS_TSWVL Non-structural protein NS-S

1 entry

Tomato spotted wilt virus (TSWV)

GP_TSWV Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G2); ...

1 entry

Tomato spotted wilt virus (isolate D) (TSWV)

GP_TSWVD Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G2); ...

1 entry

Tomato spotted wilt virus (strain Brazilian Br-03) (TSWV) (Tomato chlorotic spot virus)

NCAP_TSWV3 Nucleoprotein (Nucleocapsid protein) (Protein N)

1 entry

Tomato spotted wilt virus (strain Hawaiian) (TSWV)

NCAP_TSWVH Nucleoprotein (Nucleocapsid protein) (Protein N)

1 entry

Tomato spotted wilt virus (strain Regular2A) (TSWV)

GP_TSWVR Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G2); ...

1 entry

Tomato spotted wilt virus (strain SA-05) (TSWV) (Groundnut ringspot virus)

NCAP_TSWVS Nucleoprotein (Nucleocapsid protein) (Protein N)
These structures were created with the help of Colabfold by Jason Nomburg, Nathan Price and Jennifer Doudna (ModelArchive).

Bean necrotic mosaic virus taxid:1033976

Protein ModelArchive
Envelopment polyprotein (M polyprotein) ma-jd-viral-30783
Non-structural movement protein ma-jd-viral-27548
Non-structural silencing protein ma-jd-viral-47173
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-09322

Capsicum chlorosis virus taxid:163325

Protein ModelArchive
Envelopment polyprotein (M polyprotein) ma-jd-viral-30784
Non-structural protein ma-jd-viral-27539
Non-structural protein ma-jd-viral-47166
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-09323

Chrysanthemum stem necrosis virus taxid:83871

Protein ModelArchive
Envelopment polyprotein (M polyprotein) ma-jd-viral-30774
Movement protein ma-jd-viral-27536
Nonstructural protein ma-jd-viral-47169
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-09315

Groundnut ringspot and Tomato chlorotic spot virus reassortant taxid:1027232

Protein ModelArchive
Envelopment polyprotein (M polyprotein) ma-jd-viral-30773
Nonstructural movement protein ma-jd-viral-27547
Nonstructural silencing protein ma-jd-viral-47170
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-09327

Melon yellow spot virus taxid:89471

Protein ModelArchive
Envelopment polyprotein (M polyprotein) ma-jd-viral-30770
Nonstructural protein ma-jd-viral-27544

Pepper chlorotic spot virus taxid:1414655

Protein ModelArchive
Envelopment polyprotein (M polyprotein) ma-jd-viral-30765
Nonstructural NSm protein ma-jd-viral-27541
Nonstructural protein ma-jd-viral-47180
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-09321

Tomato zonate spot virus taxid:460926

Protein ModelArchive
Envelopment polyprotein (M polyprotein) ma-jd-viral-30776
NSm (Non-structural movement protein) (Nonstructural movement protein) ma-jd-viral-27543
NSs ma-jd-viral-47168
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-09319

Tospovirus kiwifruit/YXW/2014 taxid:1857323

Protein ModelArchive
Envelopment polyprotein (M polyprotein) ma-jd-viral-30775
NSs ma-jd-viral-47179
Nsm ma-jd-viral-27530
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-09325

Zucchini lethal chlorosis virus taxid:83872

Protein ModelArchive
Envelopment polyprotein (M polyprotein) ma-jd-viral-30772
Movement protein NSm ma-jd-viral-27531
NSs non-structural protein ma-jd-viral-47177
Nucleoprotein (Nucleocapsid protein) ma-jd-viral-09326