Molecular biology
VIRION
Icosahedral pseudo T=21 dextro capsid 59 nm in diameter.
- Double jelly roll-fold major capsid protein [Hexon]
- Single jelly roll-fold penton capsid protein [Penton]
GENOME
Circular, single-stranded DNA genome of about 9.2kb encoding for 16 proteins.
GENE EXPRESSION
ENZYMES
REPLICATION
- Adsorption: the phage attach to target cell
- Injection: viral DNA is injected in host cell cytoplasm after fusion of viral membrane with host membrane.
- Transcription and translation of early genes
- Replication of genomic DNA via a rolling-circle mechanism
- Transcription and translation of late genes
- Capsid proteins polymerize around a lipoprotein vesicle translocated in the cytoplasm by virion assembly factors.
- Genomic DNA is packaged in new virions
- Mature virions are released from the cell by lysis