Microviridae (taxid:10841)
VIRION
Non-enveloped, round, T=1 icosahedral symmetry, about 30 nm in diameter. The capsid consists of 12 pentagonal trumpet-shaped pentomers.
The virion is composed of 60 copies each of the F, G, and J proteins, and 12 copies of the H protein. There are 12 spikes which are each composed of 5 G and one H proteins.
GENOME
Circular, ssDNA(+) genome of 4.4 to 6.1kb. replication occurs via dsDNA intermediate and rolling circle.
GENE EXPRESSION
Early and late genes promoters tightly regulate the timing of gene expression, which is crucial for the replication cycle.
ENZYMES
REPLICATION
CYTOPLASMIC
- The viral particle attaches to host cell by binding host lipopolysaccharides.
- The proteins of the capsid perform Injection of the viral DNA through bacterial membranes into cell cytoplasm.
- Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA I (RF-I).
- Early viral genes are transcribed by host RNA polymerase, producing viral replication proteins.
- Viral protein A cleaves RF-I(+) DNA strand at the origin of replication and covalently attaches itself to the DNA.
- (+)strand replication occurs by rolling circle, which is converted to dsDNA by host polymerase, generating RF-II molecules (amplification of RF-I).
- Late viral genes are transcribed by host RNA polymerase.
- Procapsid assembly in the cytoplasm.
- Viral protein C binds to replication complex, inducing synthesis and packaging of neo-synthesized (+)ssDNA genomes (RF-III) into procapsids.
- Procapsids maturation occurs in host cytoplasm
- Mature virions are released from the cell by lysis.
Matching UniProtKB/Swiss-Prot entries
(all links/actions below point to uniprot.org website)78 entries grouped by protein
3 entries
Internal scaffolding protein ORF3
4 entries
Protein ORF5
8 entries
Capsid protein F (F protein) (GPF)
5 entries
Major spike protein G (G protein) (GPG)
4 entries
Minor spike protein H (H protein) (Pilot protein)
7 entries
DNA-binding protein J (J protein) (Small core protein)
1 entry
Protein K
2 entries
Lysis protein (E protein) (GPE)
1 entry
Uncharacterized protein N
2 entries
Uncharacterized protein ORF6
2 entries
Uncharacterized protein ORF7
1 entry
Uncharacterized protein ORF9
3 entries
Replication-associated protein ORF2 (EC 3.1.21.-) (EC 6.5.1.1) (Rep)
1 entry
Replication-associated protein A (EC 3.1.21.-) (EC 6.5.1.1) (RepA)
5 entries
Internal scaffolding protein B (EC 3.4.-.-) (Scaffolding protein B) (GPB)
5 entries
External scaffolding protein D (Scaffolding protein D) (GPD)
4 entries
A protein (GPA)
4 entries
C protein
3 entries
Lysis protein (E protein) (GPE)
4 entries
Minor spike protein (H protein) (Pilot protein)
1 entry
Small core protein (J protein)
4 entries
K protein
1 entry
Uncharacterized protein W
1 entry
Uncharacterized protein X
1 entry
Uncharacterized protein Y
1 entry