Torovirus

VIRION

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Enveloped, spherical, about 120 to 140nm in diameter. The tubular nucleocapsid is bend into an open torus hence the name "torovirus".

GENOME

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Monopartite, linear ssRNA(+) genome of 28kb in size. Capped, and polyadenylated.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Genomic RNA encodes for ORF1a, as for ORF1b, it is translated by ribosomal frameshifting. Resulting proteins pp1a and pp1ab are processed into the viral polymerase (RdRp) and other non-structural proteins involved in RNA synthesis. Structural proteins are expressed as subgenomic RNAs. Each RNA (genomic and subgenomic) is translated to yield only the protein encoded by the 5'-most ORF.
A putative 30K protein overlapping ORF1a might be expressed from an alternative CUG codon .

REPLICATION

CYTOPLASMIC

  1. Attachement of the viral S protein (maybe also HE if present) to host receptors mediates endocytosis of the virus into the host cell.
  2. Synthesis and proteolytic cleavage of the replicase polyprotein.
  3. Fusion of virus membrane with the endosomal membrane (probably mediated by E2), ssRNA(+) genome is released into the cytoplasm.
  4. Synthesis and proteolytic cleavage of the replicase polyprotein.
  5. Replication occurs in viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  6. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  7. Synthesis of structural proteins encoded by subgenomic mRNAs.
  8. Assembly and budding at membranes of the endoplasmic reticulum (ER), the intermediate compartments, and/or the Golgi complex.
  9. Release of new virions by exocytosis.

Host-virus interaction

Apoptosis modulation

Equine torovirus Berne virus induces apoptosis in infected cells at late times postinfection via host caspases activation .