Hepeviridae (taxid:3079366)

VIRION

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Quasi-enveloped, spherical, about 32-34 nm in diameter. The RNA genome is enclosed within a capsid that is composed of 180 capsid proteins, assembled into a T=3 icosahedral particle . The HEV particles present in feces and bile are non-enveloped, while those in circulating blood and culture supernatants are covered with a cellular membrane, similar to enveloped viruses.
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GENOME

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Monopartite, linear, ssRNA(+) genome of 7.2 kb. The 5' end is capped and the 3' terminus is polyadenylated. Their is no VPg at the 5'-terminus of the genome. The genome consists of three partially overlapping ORFs.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. ORF 1 encodes the nonstructural proteins, it is not clear whether this polyprotein is cleaved or not; ORF 2 encodes the capsid protein; ORF 3 encodes a small immunogenic protein. ORF2 is translated by leaky scanning from the bicistronic ORF3-2 subgenomic RNA.

ENZYMES

REPLICATION

  1. Attachement of the virus to host receptors mediates internalization into the host cell by clathrin-mediated endocytosis.
  2. RNA genome is released into the cytoplasm.
  3. The positive-sense genomic ssRNA is translated into a processed ORF1 polyprotein to yield the mature non-structural proteins involved in RNA transcription.
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. Expression of two other messenger RNAs, a 3.7 kb subgenomic one and a 2.0 kb coterminal one.
  7. Virus assembly and release.

Host-virus interaction

Innate immune response inhibition

HEV ORF3 protein suppresses host interferon-α signaling by inhibiting STAT1 phosphorylation .

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

31 entries grouped by protein

10 entries

Pro-secreted protein ORF2 (Protein ORF2) (pORF2)

Select_allDeselect_all
CAPSD_AHEV
Avian hepatitis E virus (isolate Chicken/California/Meng) (AHEV) reference strain
CAPSD_HEVCH
Hepatitis E virus genotype 1 (isolate Human/China/HeBei/1987) (HEV) reference strain
CAPSD_HEVBU
Hepatitis E virus genotype 1 (isolate Human/Burma) (HEV-1)
CAPSD_HEVCT
Hepatitis E virus genotype 4 (isolate Human/China/T1) (HEV-4) (Hepatitis E virus genotype 4 (isolate Human/China/Ct1))
CAPSD_HEVHY
Hepatitis E virus genotype 1 (isolate Human/India/Hyderabad) (HEV-1)
CAPSD_HEVME
Hepatitis E virus genotype 2 (isolate Human/Mexico) (HEV-2)
CAPSD_HEVMG
Hepatitis E virus genotype 3 (isolate Swine/United States/swUS1) (HEV-3) (Hepatitis E virus genotype 3 (isolate Swine/United States/Meng))
CAPSD_HEVMY
Hepatitis E virus genotype 1 (isolate Human/Myanmar/HEVNE8L) (HEV-1)
CAPSD_HEVPA
Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1)
CAPSD_HEVUS
Hepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3)

10 entries

Protein ORF3 (pORF3)

Select_allDeselect_all
ORF3_AHEV
Avian hepatitis E virus (isolate Chicken/California/Meng) (AHEV) reference strain
ORF3_HEVCH
Hepatitis E virus genotype 1 (isolate Human/China/HeBei/1987) (HEV) reference strain

Protein ORF3 (pORF3) (Vp13)

ORF3_HEVBU
Hepatitis E virus genotype 1 (isolate Human/Burma) (HEV-1)
ORF3_HEVCT
Hepatitis E virus genotype 4 (isolate Human/China/T1) (HEV-4) (Hepatitis E virus genotype 4 (isolate Human/China/Ct1))
ORF3_HEVHY
Hepatitis E virus genotype 1 (isolate Human/India/Hyderabad) (HEV-1)
ORF3_HEVME
Hepatitis E virus genotype 2 (isolate Human/Mexico) (HEV-2)
ORF3_HEVMG
Hepatitis E virus genotype 3 (isolate Swine/United States/swUS1) (HEV-3) (Hepatitis E virus genotype 3 (isolate Swine/United States/Meng))
ORF3_HEVMY
Hepatitis E virus genotype 1 (isolate Human/Myanmar/HEVNE8L) (HEV-1)
ORF3_HEVPA
Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1)
ORF3_HEVUS
Hepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3)

1 entry

Protein ORF4

ORF4_HEVPA
Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1)

10 entries

Non-structural polyprotein pORF1

Select_allDeselect_all
POLN_AHEV
Avian hepatitis E virus (isolate Chicken/California/Meng) (AHEV) reference strain
POLN_HEVCH
Hepatitis E virus genotype 1 (isolate Human/China/HeBei/1987) (HEV) reference strain
POLN_HEVBU
Hepatitis E virus genotype 1 (isolate Human/Burma) (HEV-1)
POLN_HEVCT
Hepatitis E virus genotype 4 (isolate Human/China/T1) (HEV-4) (Hepatitis E virus genotype 4 (isolate Human/China/Ct1))
POLN_HEVHY
Hepatitis E virus genotype 1 (isolate Human/India/Hyderabad) (HEV-1)
POLN_HEVME
Hepatitis E virus genotype 2 (isolate Human/Mexico) (HEV-2)
POLN_HEVMG
Hepatitis E virus genotype 3 (isolate Swine/United States/swUS1) (HEV-3) (Hepatitis E virus genotype 3 (isolate Swine/United States/Meng))
POLN_HEVMY
Hepatitis E virus genotype 1 (isolate Human/Myanmar/HEVNE8L) (HEV-1)
POLN_HEVPA
Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1)
POLN_HEVUS
Hepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3)
These structures were created with the help of Colabfold by Jason Nomburg, Nathan Price and Jennifer Doudna (ModelArchive).

Chirohepevirus eptesici taxid:1678146

Protein ModelArchive
Pro-secreted protein ORF2 (pORF2) (Protein ORF2) ma-jd-viral-06987
Uncharacterized protein ma-jd-viral-49790

Cutthroat trout virus taxid:1016879

Protein ModelArchive
Phosphoprotein ma-jd-viral-60336
Polyprotein ma-jd-viral-34729
Polyprotein ma-jd-viral-36486
Polyprotein ma-jd-viral-60722
Pro-secreted protein ORF2 (pORF2) (Protein ORF2) ma-jd-viral-33613

Hepatitis E virus genotype 1 (isolate Human/China/HeBei/1987) taxid:652674

Protein ModelArchive
Pro-secreted protein ORF2 (Protein ORF2) (pORF2) ma-jd-viral-06986