Inoviridae

VIRION

Non-enveloped, rod of filaments of 7nm in diameter and 700 to 2000nm in length. Helical capsid with adsorption proteins on one end.

GENOME

Circular, ssDNA genome (+) of 4.5 to 8kb encoding for 4 to 10 proteins. Replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Each gene is transcribed by host cellular machinery, via a specific promoter. Some genes end by a transcription terminator.





REPLICATION

CYTOPLASMIC

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane.
  2. The proteins of the capsid perform the injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

replication cycle

  1. Viral g3p protein mediates pilus-mediated adsorption of the virus onto host cell. Pilus retraction pulls the virion to the host internal membrane.
  2. The proteins of the capsid mediate the Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA (RF).
  4. dsDNA transcription by host RNA polymerase gives rise to viral mRNAs.
  5. Viral g2p protein nicks RF DNA strand at the origin of replication.
  6. (+) strand replication occurs by rolling circle.
  7. New (+)ssDNA genomes are converted into new RF molecules, and further transcription occurs.
  8. When enough g5p protein is synthesized, conversion into RF dsDNA is inhibited, as neo-synthesized genomic ssDNA is covered with g5p.
  9. g5p are replaced by g8p proteins to trigger the assembly of the viral capsid.
  10. New virions are secreted from host cell.
  11. Infected cells continue to divide and produce virions indefinitely.

Matching UniProtKB/Swiss-Prot entries

87 entries grouped by protein (browse by keywords)

12 entries

Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein)

Select_allDeselect_all
CAPSD_BPI22
Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain
CAPSD_BPIF1
Enterobacteria phage If1 (Bacteriophage If1) reference strain
CAPSD_BPIKE
Enterobacteria phage IKe (Bacteriophage IKe) reference strain
CAPSD_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain

Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (M13 procoat) (Major coat protein)

CAPSD_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain
CAPSD_BPPF3
Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain

Capsid protein G8P (Coat protein B) (Gene 8 protein) (G8P) (Major coat protein) (Pf3 coat protein)

CAPSD_BPPHL
Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain
CAPSD_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
CAPSD_BPFD
Enterobacteria phage fd (Bacteriophage fd)
CAPSD_BPH75
Thermus phage PH75 (Bacteriophage PH75)
CAPSD_BPXF
Xanthomonas phage Xf (Bacteriophage Xf)
CAPSD_BPZJ2
Enterobacteria phage ZJ/2 (Bacteriophage ZJ-2)

7 entries

Gene 1 protein (G1P)

Select_allDeselect_all
G1P_BPI22
Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain
G1P_BPIF1
Enterobacteria phage If1 (Bacteriophage If1) reference strain
G1P_BPIKE
Enterobacteria phage IKe (Bacteriophage IKe) reference strain
G1P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G1P_BPPHL
Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain
G1P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
G1P_BPFD
Enterobacteria phage fd (Bacteriophage fd)

9 entries

Attachment protein G3P (Gene 3 protein) (G3P) (Minor coat protein)

Select_allDeselect_all
G3P_BPI22
Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain
G3P_BPIF1
Enterobacteria phage If1 (Bacteriophage If1) reference strain
G3P_BPIKE
Enterobacteria phage IKe (Bacteriophage IKe) reference strain
G3P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G3P_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain
G3P_BPPF3
Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain
G3P_BPPHL
Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain
G3P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
G3P_BPFD
Enterobacteria phage fd (Bacteriophage fd)

6 entries

Virion export protein (Gene 4 protein) (G4P)

Select_allDeselect_all
G4P_BPI22
Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain
G4P_BPIF1
Enterobacteria phage If1 (Bacteriophage If1) reference strain
G4P_BPIKE
Enterobacteria phage IKe (Bacteriophage IKe) reference strain
G4P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G4P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
G4P_BPFD
Enterobacteria phage fd (Bacteriophage fd)

9 entries

DNA-Binding protein G5P (G5P) (GPV) (Single-stranded DNA-binding protein)

Select_allDeselect_all
G5P_BPI22
Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain

DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)

G5P_BPIF1
Enterobacteria phage If1 (Bacteriophage If1) reference strain
G5P_BPIKE
Enterobacteria phage IKe (Bacteriophage IKe) reference strain
G5P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain

DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)

G5P_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain
G5P_BPPF3
Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain

DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)

G5P_BPPHL
Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain

DNA-Binding protein G5P (G5P) (Single-stranded DNA-binding protein)

G5P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
G5P_BPFD
Enterobacteria phage fd (Bacteriophage fd)

8 entries

Head virion protein G6P (Coat protein D) (G6P)

Select_allDeselect_all
G6P_BPIF1
Enterobacteria phage If1 (Bacteriophage If1) reference strain
G6P_BPIKE
Enterobacteria phage IKe (Bacteriophage IKe) reference strain
G6P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G6P_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain
G6P_BPPF3
Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain
G6P_BPPHL
Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain
G6P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
G6P_BPFD
Enterobacteria phage fd (Bacteriophage fd)

7 entries

Tail virion protein G7P (Coat protein C, polypeptide I) (Gene 7 protein) (G7P)

Select_allDeselect_all
G7P_BPI22
Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain
G7P_BPIF1
Enterobacteria phage If1 (Bacteriophage If1) reference strain
G7P_BPIKE
Enterobacteria phage IKe (Bacteriophage IKe) reference strain
G7P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G7P_BPPHL
Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain
G7P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
G7P_BPFD
Enterobacteria phage fd (Bacteriophage fd)

7 entries

Tail virion protein G9P (Coat protein C, polypeptide II) (G9P)

Select_allDeselect_all
G9P_BPI22
Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain
G9P_BPIF1
Enterobacteria phage If1 (Bacteriophage If1) reference strain
G9P_BPIKE
Enterobacteria phage IKe (Bacteriophage IKe) reference strain
G9P_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
G9P_BPPHL
Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain
G9P_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
G9P_BPFD
Enterobacteria phage fd (Bacteriophage fd)

1 entry

Putative integrase

INTG_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

8 entries

Replication-associated protein G2P (Rep) (EC 3.1.21.-) (EC 6.5.1.1) (G2P) (Gene 2 protein)

Select_allDeselect_all
REP_BPI22
Enterobacteria phage I2-2 (Bacteriophage I2-2) reference strain
REP_BPIF1
Enterobacteria phage If1 (Bacteriophage If1) reference strain
REP_BPIKE
Enterobacteria phage IKe (Bacteriophage IKe) reference strain
REP_BPM13
Enterobacteria phage M13 (Bacteriophage M13) reference strain
REP_BPPF3
Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain
REP_BPPHL
Xanthomonas phage phiLf (Bacteriophage phi-Lf) reference strain
REP_BPF1
Enterobacteria phage f1 (Bacteriophage f1)
REP_BPFD
Enterobacteria phage fd (Bacteriophage fd)

1 entry

3.2 kDa protein (ORF 30)

VG030_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

1 entry

6.4 kDa protein (ORF 58)

VG058_BPPF3
Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain

2 entries

7.9 kDa protein (ORF 71)

Select_allDeselect_all
VG071_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

8.6 kDa protein (ORF 71)

VG071_BPPF3
Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain

1 entry

Uncharacterized protein ORF83 (ORF083)

VG083_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

1 entry

10.1 kDa protein (ORF 90)

VG090_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

1 entry

10.9 kDa protein (ORF 96)

VG096_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

1 entry

11.2 kDa protein (ORF 103)

VG103_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

1 entry

12.0 kDa protein (ORF 110)

VG110_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

1 entry

Putative assembly protein ORF301 (ORF301)

VG301_BPPF3
Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain

1 entry

Uncharacterized protein ORF424 (ORF 424)

VG424_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

2 entries

Putative assembly protein ORF430 (ORF430)

Select_allDeselect_all
VG430_BPPF1
Pseudomonas phage Pf1 (Bacteriophage Pf1) reference strain

Uncharacterized protein ORF430 (ORF430)

VG430_BPPF3
Pseudomonas phage Pf3 (Bacteriophage Pf3) reference strain