Entomopoxvirinae (taxid:10284)
VIRION

Enveloped, brick-shaped or ovoid virion, 70-250x350nm in size. Two distinct infectious virus particles exists: the intracellular mature virus (IMV) and the extracellular enveloped virus (EEV).
GENOME
Linear, dsDNA genome of 250-380kb. The linear genome is flanked by inverted terminal repeat (ITR) sequences which are covalently-closed at their extremities.
GENE EXPRESSION
ENZYMES
- DNA dependent DNA polymerase
- DNA dependent RNA polymerase
- Cell-type capping
- RNA TPase
- GTase
- N7MTase
- 2'O methylase
- Core protease (Peptidase C57)
- Metalloprotease (Peptidase M44)
REPLICATION
CYTOPLASMIC
- Attachment of the viral proteins to host glycosaminoglycans (GAGs) mediates endocytosis of the virus into the host cell.
- Fusion with the plasma membrane to release the core into the host cytoplasm.
- Early phase: early genes are transcribed in the cytoplasm by viral RNA polymerase. Early expression begins at 30 minutes post-infection.
- Core is completely uncoated as early expression ends, viral genome is now free in the cytoplasm.
- Intermediate phase: Intermediate genes are expressed, triggering genomic DNA replication at approximately 100 minutes post-infection.
- Late phase: Late genes are expressed from 140 min to 48 hours post-infection, producing all structural proteins.
- Assembly of progeny virions starts in cytoplasmic viral factories, producing an spherical immature particle. This virus particle matures into brick-shaped intracellular mature virion (IMV).
- IMV virion can be released upon cell lysis, or can acquire a second double membrane from trans-Golgi and bud as external enveloped virion (EEV).
- Mature virion can be occluded in spheroids comprised of spheroidin protein.
Host-virus interaction
Apoptosis modulation
The Amsacta moorei entomopoxvirus encodes AMV p33, a functional homolog of the baculovirus apoptosis inhibitor p35 . P33 is a direct inhibitor of caspases.
Matching UniProtKB/Swiss-Prot entries
(all links/actions below point to uniprot.org website)24 entries grouped by protein
1 entry
Envelope protein A28 homolog (Protein G4R)
1 entry
DNA polymerase (EC 2.7.7.7)
1 entry
Early transcription factor 70 kDa subunit (EC 3.6.4.-) (ATP-dependent helicase VETFS) (ETF small subunit)
1 entry
Early transcription factor 82 kDa subunit (ETF large subunit)
1 entry
Core protease I7 homolog (EC 3.4.22.-)
3 entries
Thymidine kinase (EC 2.7.1.21)
2 entries
Putative glycylpeptide N-tetradecanoyltransferase (EC 2.3.1.97) (Myristoyl-CoA:protein N-myristoyltransferase) (NMT) (Peptide N-myristoyltransferase)
1 entry
RNA helicase NPH-II (EC 3.6.4.13) (Nucleoside triphosphatase II) (NTPase II) (Nucleoside triphosphate phosphohydrolase II) (NPH II)
5 entries
Nucleoside triphosphatase I (EC 3.6.1.15) (NPH-I) (Nucleoside triphosphate phosphohydrolase I) (NPH I)
1 entry
Putative tyrosine-protein phosphatase AMV078 (EC 3.1.3.48)
1 entry
Spheroidin
2 entries
Spindolin (Fusolin) (p50)
1 entry
Uncharacterized protein AMV183/G2R
1 entry
Uncharacterized protein AMV185/G3L
1 entry
Uncharacterized leucine-rich repeat-containing protein AMV014/Q3
1 entry