Mastadenovirus

VIRION

Non-enveloped capsid with a pseudo T=25 icosahedral symmetry . The capsid diameter is about 90 nm. The capsid shell consists of 720 hexon subunits arranged as 240 trimers and 12 vertex penton capsomers each with a fiber protruding from the surface.


GENOME

Non segmented, linear double-stranded DNA of 35-36kb encoding about 40 proteins. The genome has terminally redundant sequences which have inverted terminal repetitions (ITR). The terminal protein (TP) is covalently attached to each end of the genome.

GENE EXPRESSION

Transcription is nuclear, in three phases; early, intermediate (replication), and late (virion assembly). All genes are transcribed by host RNA pol II except virus-associated (VA) gene(s) of some primate adenoviruses transcribed by RNA pol III. Genes transcribed by RNA pol II give rise to multiple mRNA that are produced by alternative splicing and use of different poly(A) sites.

REPLICATION

NUCLEUS

  1. Attachment of the viral fibers to the host CAR adhesion receptor. Subsequent binding of the penton protein to host integrin entry receptors mediates internalization into the host cell by clathrin-mediated endocytosis of the virus and fiber shedding. Some serotypes also seem to use macropinocytosis.
  2. Disruption of host endosomal membrane by lytic protein VI releases the viral capsid in the cytosol.
  3. Microtubular transport toward nucleus of the viral genome still protected by the core protein VII and a partial capsid mainly composed of hexons and protein IX.
  4. Docking at the NPC and capsid disruption.
  5. Import of the viral genome into host nucleus mediated by core protein VII.
  6. Transcription of early genes (E genes) by host RNA pol II: these proteins optimize the cellular milieu for viral replication, and counteract a variety of antiviral defenses.
  7. Intermediate genes activate replication of the DNA genome by DNA strand displacement in the nucleus.
  8. Expression of L4-22K and L4-33K causes early to late switch. Transcription of late genes (L genes) by host RNA pol II, mostly encoding structural proteins.
  9. Host translation shutoff performed by the viral 100K protein.
  10. Assembly of new virions in the nucleus.
  11. Virions are released by lysis of the cell.
  12. Virion maturation by the viral protease.

Host-virus interaction

Adaptive immune response inhibition

The adenovirus E19 protein downregulates cell-surface expression of MHC-I by binding and retains the heavy chain in ER. E19 binds TAP and acts as a tapasin inhibitor, preventing class I/TAP association .

Apoptosis modulation

Soon after infection, the expression of E1A protein drives the cell cycle into S-phase so that the virus can replicate its genome optimally . However, this arrest leads to the accumulation of host p53 protein and subsequently apoptosis. The apoptosis is then inhibited by different viral proteins including E1B 55K, E4 orf6 or the bcl-2 homolog E1B 19K .

Autophagy modulation

Autophagy is triggered after adenoviral infection and may facilitate viral replication and/or cell lysis. The E1B 19K viral protein interacts with host Beclin 1, removes Bcl-2 (a negative regulator of autophagy) from Beclin-1 and thereby promotes autophagy .

Cell-cycle modulation

The expression of E1A protein drives the cell cycle into S-phase so that the virus can replicate its genome optimally .

Innate immune response inhibition

Adenoviruses do not inhibit the cascade leading to production of interferon-beta directly. However, they evade the host innate immunity by targeting a cellular histone modification. Indeed, the E1A viral protein blocks the IFN-induced H2B mono-ubiquitination and thereby associated ISGs expression .

Matching UniProtKB/Swiss-Prot entries

139 entries grouped by strain (browse by keywords)

Only complete proteome entries are shown (view all 326 entries)

45 entries

Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2) reference strain

Select_allDeselect_all
CAP6_ADE02 Pre-protein VI (pVI) [Cleaved into: Endosome lysis protein; Protease cofactor (pVI-C)]
ADP_ADE02 Adenovirus death protein (ADP) (Early-3 11.6 kDa glycoprotein) (E3-11.6K)
COR10_ADE02 Pre-core protein X (pX) (11 kDa core protein) (Protein mu) (pMu) [Cleaved into: Core protein X]
Y112_ADE02 Uncharacterized protein F-112
Y168_ADE02 Uncharacterized protein C-168
Y172_ADE02 Uncharacterized protein D-172
Y215_ADE02 Uncharacterized protein F-215
Y115_ADE02 Uncharacterized protein E-115
SF33K_ADE02 Protein 33K (L4-33K) (Splicing factor 33K) (Terminase, small subunit)
Y137_ADE02 Uncharacterized protein B-137
Y116_ADE02 Uncharacterized 11.6 kDa early protein
Y121_ADE02 Uncharacterized protein F-121
UXP_ADE02 U exon protein (UXP)
Y95_ADE02 Uncharacterized protein E-95
SPIKE_ADE02 Fiber protein (SPIKE) (Protein IV)
PKG2_ADE02 Packaging protein 2 (Packaging protein 22K) (L4-22K)
PRO_ADE02 Protease (EC 3.4.22.39) (Adenain) (Adenovirus protease) (AVP) (Adenovirus proteinase) (Endoprotease)
TERM_ADE02 Preterminal protein (pTP) (Bellett protein) (Precursor terminal protein) [Cleaved into: Intermediate ...
SHUT_ADE02 Shutoff protein (100 kDa protein) (p100K) (100K-chaperone protein) (L4-100K) (Shutoff protein 100K)
E1A_ADE02 Early E1A protein (Early E1A 32 kDa protein)
CAPSH_ADE02 Hexon protein (CP-H) (Protein II)
LEAD_ADE02 I-leader protein
PKG1_ADE02 Packaging protein 1 (Packaging protein IVa2)
NP_ADE02 Pre-histone-like nucleoprotein (Pre-core protein VII) (pVII) [Cleaved into: Histone-like ...
PKG3_ADE02 Packaging protein 3 (L1-52/55 kDa protein) (Packaging protein 52K)
E312_ADE02 Early E3A 12.5 kDa protein (E3-12,5K)
E4RF3_ADE02 Early 4 ORF3 protein (E4-ORF3) (E4 ORF3 control protein) (Early 4 11 kDa protein) (E4-11k)
E3145_ADE02 Early 3 14.7 kDa protein (E3-14.7k)
E4RF1_ADE02 Early 4 ORF1 protein (E4-ORF1) (E4 ORF1 control protein)
E4RF2_ADE02 Early 4 ORF2 protein (E4-ORF2)
E4RF5_ADE02 Early 4 ORF6/7 control protein (E4-ORF6/7) (Early E4 17 kDa protein)
E3RDB_ADE02 Early 3 receptor internalization and degradation beta protein (E3 RID-beta protein) (Early E3B 14.5 ...
CORE5_ADE02 Core-capsid bridging protein (Core protein V)
CAP8_ADE02 Pre-hexon-linking protein VIII (Pre-protein VIII) (pVIII) [Cleaved into: Hexon-linking protein-N ...
E434_ADE02 Early 4 ORF6 protein (E4-ORF6) (Early 4 34 kDa protein) (E4-34k)
DNB2_ADE02 DNA-binding protein (DBP) (Early 2A protein) (Early E2A DNA-binding protein)
E3RDA_ADE02 Pre-early 3 receptor internalization and degradation alpha protein (Pre-E3-RID-alpha protein) ...
CAP9_ADE02 Hexon-interlacing protein (Protein IX)
DPOL_ADE02 DNA polymerase (EC 2.7.7.7)
E4RF4_ADE02 Early 4 ORF4 protein (E4 ORF4) (Early E4 13 kDa protein)
E3GL_ADE02 Early E3 18.5 kDa glycoprotein (E3-19K) (E3gp 19 kDa) (E19) (GP19K)
CAPSP_ADE02 Penton protein (CP-P) (Penton base protein) (Protein III)
CAP3_ADE02 Pre-hexon-linking protein IIIa (Capsid vertex-specific component IIIa) (CVSC) (Protein IIIa) (pIIIa) ...
E1BS_ADE02 E1B protein, small T-antigen (E1B 19 kDa protein) (E1B-19K) (E1B-175R)
E1B55_ADE02 E1B 55 kDa protein (E1B-55K) (E1B protein, large T-antigen) (E1B-495R)

30 entries

Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5) reference strain

Select_allDeselect_all
CAP9_ADE05 Hexon-interlacing protein (Protein IX)
TERM_ADE05 Preterminal protein (pTP) (Bellett protein) (Precursor terminal protein) [Cleaved into: Intermediate ...
PKG1_ADE05 Packaging protein 1 (Packaging protein IVa2)
SF33K_ADE05 Protein 33K (L4-33K) (Splicing factor 33K) (Terminase, small subunit)
SPIKE_ADE05 Fiber protein (SPIKE) (Protein IV)
UXP_ADE05 U exon protein (UXP)
SHUT_ADE05 Shutoff protein (100 kDa protein) (p100K) (100K-chaperone protein) (L4-100K) (Shutoff protein 100K)
PKG2_ADE05 Packaging protein 2 (Packaging protein 22K) (L4-22K)
NP_ADE05 Pre-histone-like nucleoprotein (Pre-core protein VII) (pVII) [Cleaved into: Histone-like ...
E411_ADE05 Probable early E4 11 kDa protein
E310_ADE05 Early E3B 10.4 kDa protein
CAPSP_ADE05 Penton protein (CP-P) (Penton base protein) (Protein III)
PRO_ADE05 Protease (EC 3.4.22.39) (Adenain) (Adenovirus protease) (AVP) (Adenovirus proteinase) (Endoprotease)
CAP3_ADE05 Pre-hexon-linking protein IIIa (Capsid vertex-specific component IIIa) (CVSC) (Protein IIIa) (pIIIa) ...
PKG3_ADE05 Packaging protein 3 (L1-52/55 kDa protein) (Packaging protein 52K)
DNB2_ADE05 DNA-binding protein (DBP) (Early 2A protein) (Early E2A DNA-binding protein)
E312_ADE05 Early E3A 12.5 kDa protein
E3145_ADE05 Early E3 14.5 kDa protein
E3B14_ADE05 Early E3B 14.6 kDa protein
CORE5_ADE05 Core-capsid bridging protein (Core protein V)
COR10_ADE05 Pre-core protein X (pX) (11 kDa core protein) (Protein mu) (pMu) [Cleaved into: Core protein X]
CAP8_ADE05 Pre-hexon-linking protein VIII (Pre-protein VIII) (pVIII) [Cleaved into: Hexon-linking protein-N ...
E311_ADE05 Early E3A 10.5 kDa glycoprotein
E3GL_ADE05 Early E3 18.5 kDa glycoprotein (E3-19K) (E3gp 19 kDa) (E19) (GP19K)
DPOL_ADE05 DNA polymerase (EC 2.7.7.7)
CAP6_ADE05 Pre-protein VI (pVI) [Cleaved into: Endosome lysis protein; Protease cofactor (pVI-C)]
E1A_ADE05 Early E1A protein (Early E1A 32 kDa protein)
CAPSH_ADE05 Hexon protein (CP-H) (Protein II)
E1BS_ADE05 E1B protein, small T-antigen (E1B 19 kDa protein) (E1B-19K)
E1B55_ADE05 E1B 55 kDa protein (E1B-55K) (E1B protein, large T-antigen) (E1B-495R)

26 entries

Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12) reference strain

Select_allDeselect_all
CAPSH_ADE12 Hexon protein (CP-H) (Protein II)
CAP8_ADE12 Pre-hexon-linking protein VIII (Pre-protein VIII) (pVIII) [Cleaved into: Hexon-linking protein-N ...
CAP3_ADE12 Pre-hexon-linking protein IIIa (Capsid vertex-specific component IIIa) (CVSC) (Protein IIIa) (pIIIa) ...
SPIKE_ADE12 Fiber protein (SPIKE) (Protein IV)
SHUT_ADE12 Shutoff protein (100 kDa protein) (p100K) (100K-chaperone protein) (L4-100K) (Shutoff protein 100K)
TERM_ADE12 Preterminal protein (pTP) (Bellett protein) (Precursor terminal protein) [Cleaved into: Intermediate ...
E310_ADE12 Early E3B 10.4 kDa protein
DNB2_ADE12 DNA-binding protein (DBP) (Early 2A protein) (Early E2A DNA-binding protein)
E312_ADE12 Early E3A 12.1 kDa protein
E3B14_ADE12 Early E3B 12.7 kDa protein
E434_ADE12 Early E4 34 kDa protein
DPOL_ADE12 DNA polymerase (EC 2.7.7.7)
PKG1_ADE12 Packaging protein 1 (Packaging protein IVa2)
L2MU_ADE12 Late L2 mu core protein (11 kDa core protein) (Protein X) (pX) (pMu)
PRO_ADE12 Protease (EC 3.4.22.39) (Adenain) (Adenovirus protease) (AVP) (Adenovirus proteinase) (Endoprotease)
PKG3_ADE12 Packaging protein 3 (L1-52/55 kDa protein) (Packaging protein 52K)
E1BS_ADE12 E1B protein, small T-antigen (E1B 19 kDa protein) (E1B-19K)
CAP9_ADE12 Hexon-interlacing protein (Protein IX)
DNBI_ADE12 Probable DNA-binding protein (Agnoprotein)
E411_ADE12 Probable early E4 11 kDa protein
CAPSP_ADE12 Penton protein (CP-P) (Penton base protein) (Protein III)
E1A_ADE12 Early E1A protein (Early E1A 29.5 kDa protein)
E413_ADE12 Probable early E4 13 kDa protein
CAP6_ADE12 Pre-protein VI (pVI) [Cleaved into: Endosome lysis protein; Protease cofactor (pVI-C)]
E1B55_ADE12 E1B 55 kDa protein (E1B-55K) (E1B protein, large T-antigen) (E1B-495R)
CORE5_ADE12 Core-capsid bridging protein (Core protein V)

6 entries

Porcine adenovirus A serotype 3 (PAdV-3) (Porcine adenovirus 3) reference strain

Select_allDeselect_all
CAPSH_ADEP3 Hexon protein (CP-H) (Protein II)
CAP8_ADEP3 Pre-hexon-linking protein VIII (Pre-protein VIII) (pVIII) [Cleaved into: Hexon-linking protein-N ...
SPIKE_ADEP3 Fiber protein (SPIKE) (Protein IV)
PRO_ADEP3 Protease (EC 3.4.22.39) (Adenain) (Adenovirus protease) (AVP) (Adenovirus proteinase) (Endoprotease)
SHUT_ADEP3 Shutoff protein (100 kDa protein) (p100K) (100K-chaperone protein) (L4-100K) (Shutoff protein 100K)
CAPSP_ADEP3 Penton protein (CP-P) (Penton base protein) (Protein III)

3 entries

Bovine adenovirus 2 (BAdV-2) (Mastadenovirus bos2) reference strain

Select_allDeselect_all
CAP8_ADEB2 Pre-hexon-linking protein VIII (Pre-protein VIII) (pVIII) [Cleaved into: Hexon-linking protein-N ...
TERM_ADEB2 Preterminal protein (pTP) (Bellett protein) (Precursor terminal protein) [Cleaved into: Intermediate ...
DPOL_ADEB2 DNA polymerase (EC 2.7.7.7)

3 entries

Bovine adenovirus B serotype 3 (BAdV-3) (Mastadenovirus bos3) reference strain

Select_allDeselect_all
SPIKE_ADEB3 Fiber protein (SPIKE) (Protein IV)
E3145_ADEB3 Early E3 14.5 kDa protein
PRO_ADEB3 Protease (EC 3.4.22.39) (Adenain) (Adenovirus protease) (AVP) (Adenovirus proteinase) (Endoprotease)

1 entry

Human adenovirus D serotype 17 (HAdV-17) (Human adenovirus 17) reference strain

PRO_ADE17 Protease (EC 3.4.22.39) (Adenain) (Adenovirus protease) (AVP) (Adenovirus proteinase) (Endoprotease)

1 entry

Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)

LEAD_ADE05 I-leader protein

20 entries

Human adenovirus F serotype 40 (HAdV-40) (Human adenovirus 40)

Select_allDeselect_all
CAPSH_ADE40 Hexon protein (CP-H) (Protein II)
SPIK1_ADE40 Fiber protein 1 (SPIKE)
SF33K_ADE40 Protein 33K (L4-33K) (Splicing factor 33K) (Terminase, small subunit)
SPIK2_ADE40 Fiber protein 2
TERM_ADE40 Preterminal protein (pTP) (Bellett protein) (Precursor terminal protein) [Cleaved into: Intermediate ...
SHUT_ADE40 Shutoff protein (100 kDa protein) (p100K) (100K-chaperone protein) (L4-100K) (Shutoff protein 100K)
PKG1_ADE40 Packaging protein 1 (Packaging protein IVa2)
NP_ADE40 Pre-histone-like nucleoprotein (Pre-core protein VII) (pVII) [Cleaved into: Histone-like ...
E1B55_ADE40 E1B 55 kDa protein (E1B-55K) (E1B protein, large T-antigen) (E1B-495R)
L2MU_ADE40 Late L2 mu core protein (Protein X) (pX) (pMu)
PRO_ADE40 Protease (EC 3.4.22.39) (Adenain) (Adenovirus protease) (AVP) (Adenovirus proteinase) (Endoprotease)
DNB2_ADE40 DNA-binding protein (DBP) (Early 2A protein) (Early E2A DNA-binding protein)
CAP9_ADE40 Hexon-interlacing protein (Protein IX)
CAP8_ADE40 Pre-hexon-linking protein VIII (Pre-protein VIII) (pVIII) [Cleaved into: Hexon-linking protein-N ...
E1BS_ADE40 E1B protein, small T-antigen (E1B 19 kDa protein) (E1B-19K)
E434_ADE40 Early E4 34 kDa protein
DPOL_ADE40 DNA polymerase (EC 2.7.7.7)
CAP6_ADE40 Pre-protein VI (pVI) [Cleaved into: Endosome lysis protein; Protease cofactor (pVI-C)]
E1A_ADE40 Early E1A protein (Early E1A 27 kDa protein)
CORE5_ADE40 Core-capsid bridging protein (Core protein V)

4 entries

Human adenovirus D serotype 9 (HAdV-9) (Human adenovirus 9)

Select_allDeselect_all
CAPSH_ADE09 Hexon protein (CP-H) (Protein II)
E4OR1_ADE09 E4-ORF1 (EC 3.6.1.23) (Early E4 14.0 kDa protein) (ORF1) (Probable dUTPase E4 ORF1)
PRO_ADE09 Protease (EC 3.4.22.39) (Adenain) (Adenovirus protease) (AVP) (Adenovirus proteinase) (Endoprotease)
E434_ADE09 Early E4 34 kDa protein