Machlomovirus (taxid:39739)

VIRION

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Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).
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GENOME

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Linear, ssRNA(+) genome of 4-5.4 kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. The ORF2 is expressed by leaky scanning of the genomic RNA. Two 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1. ORF3-RT is also expressed through suppression of termination at the end of ORF3.
Cap-independent translation is mediated possibly through the interaction between a 3'-cap-independent translation element and a 5'UTR stem-loop.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3'co-terminal subgenomic RNAs that will generate the capsid and ORF3/ORF3-RT proteins.
  7. Virus assembly in the cytoplasm.

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

5 entries grouped by protein

1 entry

Capsid protein (Coat protein) (p25)

CAPSD_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

1 entry

Protein p31

MP1_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

1 entry

p7b

MP2_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

1 entry

Uncharacterized protein p32

P32_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

1 entry

RNA-directed RNA polymerase (EC 2.7.7.48) (111 kDa protein) (Protein p111)

RDRP_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain