Alphatrevirus

VIRION

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Non-enveloped, round, T=1 icosahedral symmetry, about 30 nm in diameter. The capsid consists of 12 pentagonal trumpet-shaped pentomers. The virion is composed of 60 copies each of the F, G, and J proteins, and 12 copies of the H protein. There are 12 spikes which are each composed of 5 G and one H proteins.


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GENOME

Circular, ssDNA(+) genome of 4.4 to 6.1kb. replication occurs via dsDNA intermediate and rolling circle.

GENE EXPRESSION

Early and late genes promoters tightly regulate the timing of gene expression, which is crucial for the replication cycle.

REPLICATION

CYTOPLASMIC

  1. The viral particle attaches to host cell by binding host lipopolysaccharides.
  2. The proteins of the capsid perform Injection of the viral DNA through bacterial membranes into cell cytoplasm.
  3. Host polymerase convert the (+)ssDNA viral genome into a covalently closed dsDNA called replicative form DNA I (RF-I).
  4. Early viral genes are transcribed by host RNA polymerase, producing viral replication proteins.
  5. Viral protein A cleaves RF-I(+) DNA strand at the origin of replication and covalently attaches itself to the DNA.
  6. (+)strand replication occurs by rolling circle, which is converted to dsDNA by host polymerase, generating RF-II molecules (amplification of RF-I).
  7. Late viral genes are transcribed by host RNA polymerase.
  8. Procapsid assembly in the cytoplasm.
  9. Viral protein C binds to replication complex, inducing synthesis and packaging of neo-synthesized (+)ssDNA genomes (RF-III) into procapsids.
  10. Procapsids maturation occurs in host cytoplasm
  11. Mature virions are released from the cell by lysis.

Matching UniProtKB/Swiss-Prot entries

20 entries grouped by protein (browse by keywords)

2 entries

Capsid protein F (F protein) (GPF)

Select_allDeselect_all
CAPSD_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
CAPSD_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)

2 entries

Major spike protein G (G protein) (GPG)

Select_allDeselect_all
G_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
G_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)

2 entries

DNA-binding protein J (J protein) (Small core protein)

Select_allDeselect_all
J_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
J_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)

2 entries

Internal scaffolding protein B (EC 3.4.-.-) (Scaffolding protein B) (GPB)

Select_allDeselect_all
SCAFB_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
SCAFB_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)

2 entries

External scaffolding protein D (Scaffolding protein D) (GPD)

Select_allDeselect_all
SCAFD_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
SCAFD_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)

2 entries

A protein (GPA)

Select_allDeselect_all
VGA_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
VGA_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)

2 entries

C protein

Select_allDeselect_all
VGC_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
VGC_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)

2 entries

Lysis protein (E protein) (GPE)

Select_allDeselect_all
VGE_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
VGE_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)

2 entries

Minor spike protein (H protein) (Pilot protein)

Select_allDeselect_all
VGH_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
VGH_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)

2 entries

K protein

Select_allDeselect_all
VGK_BPAL3
Escherichia phage alpha3 (Bacteriophage alpha-3) reference strain
VGK_BPPHK
Enterobacteria phage phiK (Bacteriophage phi-K)