Hartmanivirus

VIRION

Enveloped, spherical with a diameter of about 120-150nm.

GENOME

Segmented Negative-stranded RNA linear genome, L segment is about 6 kb and S segment 3.3 kb.
Encodes for three proteins.

GENE EXPRESSION

S segment is ambisens. The viral RNA dependent RNA polymerase (L) binds to a promoter on each encapsidated segment, and transcribes a messenger RNA. Transcription is terminated by a strong hairpin sequence at the end of each gene.

REPLICATION

CYTOPLASM

  1. Virus attaches to host receptors though GP glycoprotein and is endocytosed into vesicles in the host cell.
  2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid is released in the cytoplasm.
  3. Sequential transcription, viral mRNAs are capped in the cytoplasm.
  4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  5. The ribonucleocapsid buds, releasing the virion.