Spounavirinae (taxid:857473)
VIRION
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Non-enveloped, head-tail structure. The capsid is about 84-94 nm in diameter and has a T=16 icosahedral symmetry (Spounalikevirus) . The tail is 140-219 in length, contractile with globular structures at its tip, has 6 long terminal fibers, 6 short spikes and a double base plate.
GENOME
Linear, dsDNA genome of 130-160 kb, encoding for about 190-230 proteins.
GENE EXPRESSION
Genes are transcribed by operons.
ENZYMES
- DNA-directed DNA polymerase family A palm
- Cysteine protease (Peptidase C51)
- Ribonucleoside reductase
REPLICATION
CYTOPLASMIC
- Adsorption: the phage attaches to the target cell through its tail fibers.
- Ejection of the viral DNA into host cell cytoplasm by contraction of the tail sheath.
- Transcription and translation of early genes.
- Replication of viral DNA. Transcription and translation of late genes.
- Assembly of an empty procapsid and packaging of the genome.
- Viral tail fibers assembly and viral tail assembly.
- Mature virions are released from the cell by lysis.
Robert L. Duda, Roger W. Hendrix, Wai Mun Huang, James F. Conway
Curr. Biol. January 10, 2006; 16: R11-13
Matching UniProtKB/Swiss-Prot entries
(all links/actions below point to uniprot.org website)38 entries grouped by protein
1 entry
DNA polymerase (EC 2.7.7.7) (EC 3.1.11.-)
1 entry
Deoxyuridylate hydroxymethyltransferase (Deoxyuridylate hydroxymethylase) (EC 2.1.2.-) (Gp29) (dUMP hydroxymethylase) (dUMP-HMase)
1 entry
Gene 27 protein
1 entry
Gene 30 protein (GP30)
1 entry
Gene 33 protein
1 entry
Putative gene 37 protein
1 entry
Putative gene 38 protein
1 entry
Putative gene 39 protein
1 entry
Gene 40 protein
1 entry
Putative gene 41 protein
1 entry
Putative gene 42 protein
1 entry
E22 protein (Gene 43 protein)
1 entry
E3 protein (Gene 44 protein)
1 entry
Putative gene 45 protein
1 entry
Inhibitor of histone-like protein HU (Gene 46 protein) (Gp46) (Gene product 46)
1 entry
Putative gene 47 protein
1 entry
Putative gene 48 protein
1 entry
Putative gene 49 protein
1 entry
Putative gene 50 protein
1 entry
Putative gene 51 protein
1 entry
Putative gene 52 protein
1 entry
Putative gene 53 protein
1 entry
Putative gene 54 protein
1 entry
Putative gene 55 protein
1 entry
Inhibitor of host cell division protein (Gene 56 protein) (Gp56)
1 entry
Putative gene 57 protein
1 entry
Putative gene 58 protein
1 entry
Putative gene 59 protein
1 entry
Putative gene 60 protein
1 entry
5-hmdU DNA kinase (5-hydroxymethyluracil DNA kinase) (P-loop kinase) (gp186)
1 entry
DNA endonuclease I-HmuI (EC 3.1.-.-) (HNH homing endonuclease I-HmuI)
1 entry
Probable portal protein
1 entry
RNA polymerase sigma GP28 factor
1 entry
RNA polymerase sigma GP34 factor
1 entry
Thoeris anti-defense 2 (Tad2)
1 entry
Transcription factor 1
1 entry
Tail sheath protein (TSP)
1 entry