RNA termination-reinitiation (kw:KW-1158)
Ribosomal termination-reinitiation (also referred to as stop-start)
is a process which allows expression of a downstream ORF in a bicistronic mRNA. This process depends on an upstream sequence element denoted termination upstream ribosomal binding site (TURBS). In this process, ribosomes translate the upstream ORF but following termination, a proportion of 40S subunits remain tethered to the mRNA and go on to reinitiate at the start codon of the downstream ORF. This termination-dependent reinitiation strategy allows the coupled expression of products from adjacent ORFs and thus the production of a defined ratio of gene products. Host translation factor eIF3 enhances reinitiation efficiency .
Viral termination-reinitiation has been observed in vertebrates and fungus.
Tuija A. A. P?yry, Ann Kaminski, Emma J. Connell, Christopher S. Fraser, Richard J. Jackson
Genes Dev. December 1, 2007; 21: 3149-3162
Michael L. Powell, Kendra E. Leigh, Tuija A. A. P?yry, Richard J. Jackson, T. David K. Brown, Ian Brierley
PLoS ONE 2011; 6: e16822