VIRION
Unknown, discovered using viral metagenomics. Presumably enveloped.
GENOME
Segmented Negative-stranded RNA linear genome, L segment is about 10 kb, M segment about 3.5 kb and S segment 2.5 kb. Encodes at least three proteins. Enzymes: RNA dependent RNA polymerase, endonuclease? (Cap snatching).
GENE EXPRESSION
The viral RNA dependent RNA polymerase (L) binds to a promoter on each encapsidated segment, and transcribes the mRNA. These are capped by L protein during synthesis using cap snatching .
ENZYMES
REPLICATION
CYTOPLASMIC
- Virus attaches to host receptors though glycoprotein, and is endocytosed into vesicles in the host cell.
- Fusion of virus membrane with the vesicle membrane; ribonucleocapsid segments are released in the cytoplasm.
- Transcription, viral mRNAs are capped in the cytoplasm.
- Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
- The ribonucleocapsids buds, presumably releasing the virion by exocytosis.