Uukuvirus (taxid:2734594)

VIRION

Enveloped, spherical. Diameter from 80 to 120nm. Glycoproteins at the surface of the envelope are arranged on an icosahedral lattice, with T=12 symmetry.

GENOME

Segmented Negative-stranded RNA linear genome, L segment is about 6.5kb, M segment about 3.2kb and S segment about 1.7kb.
Some viruses genome comprise only Sand L segments. Encodes for two to 4 proteins.

GENE EXPRESSION

Transcription starts by viral RNA dependent RNA polymerase (L) binding to a promoter on each encapsidated segment, and is terminated by a strong hairpin sequence at the end of each gene. These are capped by L protein during synthesis using cap snatching , but are not polyadenylated.
S segment uses ambisense strategy to encode for several proteins: both genomic and antigenomic RNA are transcribed. The hairpin sequence is a stop polymerase signal which prevents ambisense transcription from producing dsRNA.

ENZYMES

• RNA-dependent RNA polymerase L
• Cap Snatching endonuclease L

REPLICATION

CYTOPLASMIC

1. Virus attaches to host receptors though Gn-Gc glycoprotein dimer, and is endocytosed into vesicles in the host cell.
2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid segments are released in the cytoplasm.
3. Transcription, viral mRNAs are capped in the cytoplasm.
4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
5. The ribonucleocapsids buds at Golgi apparatus, releasing the virion by exocytosis.