Orthotospovirus

VIRION

Enveloped, spherical. Diameter from 80 to 120nm.

GENOME

Segmented Negative-stranded RNA linear genome, L segment is about 8.8kb, M segment about 4.8kb and S segment about 3kb.
Encodes for six proteins.

GENE EXPRESSION

Transcription starts by viral RNA dependent RNA polymerase (L) binding to a promoter on each encapsidated segment, and is terminated by a strong hairpin sequence at the end of each gene. mRNAs are capped by L protein during synthesis using cap snatching , but are not polyadenylated.
S and M segments uses ambisense strategy to encode forsevral proteins: both genomic ans antigenomic RNA are transcribed. The hairpin sequence is a stop polymerase signal which prevents ambisense transcription from producing dsRNA. MRNA1 encodes for a polyprotein which is cleaved by host protease into Gn and Gc proteins.

REPLICATION

IN PLANT:CYTOPLASMIC

  1. Virus penetrates into the cell by effraction or plasmodesmata transport.
  2. Cytoplasmic transcription by viral polymerase on the RNA viral RNA template.
  3. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  4. The ribonucleocapsid can penetrate in neighboring plant cells through plasmodesmata transport
  5. Alternatively budding of particles occurs at the cell membrane, releasing new virions.

IN INSECT:CYTOPLASMIC

  1. Attachment of the viral G glycoproteins to host receptors.
  2. Fusion of virus membrane with the vesicle membrane; ribonucleocapsid is released into the cytoplasm.
  3. Cytoplasmic transcription by viral polymerase on the RNA viral RNA template.
  4. Replication presumably starts when enough nucleoprotein is present to encapsidate neo-synthetized antigenomes and genomes.
  5. Budding of particles occurs at the cell membrane, releasing new virions.

Host-virus interaction

Inhibition of host innate immunity

TSWV NSs inhibits Dicer-mediated dsRNA processing thereby inhibiting RNAi-mediated immunity to control innate immune signaling.

Matching UniProtKB/Swiss-Prot entries

7 entries grouped by strain (browse by keywords)

Only complete proteome entries are shown (view all 18 entries)

5 entries

Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV) reference strain

Select_allDeselect_all
GP_TSWV1 Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G1); ...
L_TSWV1 RNA-directed RNA polymerase L (Protein L) (EC 2.7.7.48) (Large structural protein) (Replicase) ...
NCAP_TSWV1 Nucleoprotein (Nucleocapsid protein) (Protein N)
MVP_TSWV1 Movement protein (MVP) (Non-structural movement protein) (NSm)
NSS_TSWV1 Non-structural protein NS-S

2 entries

Impatiens necrotic spot virus (INSV) reference strain

Select_allDeselect_all
GP_INSV Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G1); ...
MVP_INSV Movement protein (MVP) (Non-structural movement protein) (NSm)