Tombusviridae (taxid:39738)

VIRION

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Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion composed of 180 protein subunits).
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GENOME

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Monopartite (except Dianthovirus which is bipartite), linear, ssRNA(+) genome of 4-5.4 kb, which lacks a cap structure and a poly(A)tail.

GENE EXPRESSION

The virion RNA is infectious and serves as both the genome and viral messenger RNA. Two 5'-proximal ORFs are directly translated to produce the viral constituents of the replicase complex. RdRp is translated through suppression of termination at the end of ORF1 (tombusvirus-like and carmovirus-like), or ribosomal frameshifting (Dianthovirus and umbravirus). The downstream genes encoding the movement protein, and capsid proteins are translated from subgenomic RNAs. viral suppressor of RNA silencing (VSRs) is expressed by leaky scanning of the MVP subgenomic mRNA.
Cap-independent translation is mediated possibly through the interaction between a 3'-cap-independent translation element and a 5'UTR stem-loop.

ENZYMES

REPLICATION

CYTOPLASMIC

  1. Virus penetrates into the host cell.
  2. Uncoating, and release of the viral genomic RNA into the cytoplasm.
  3. The viral RNA is translated to produce the two proteins necessary for RNA synthesis (replication and transcription).
  4. Replication takes place in cytoplasmic viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
  5. The dsRNA genome is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
  6. The RdRp recognizes internal subgenomic promoters on the negative-sense RNA to transcribe the 3'co-terminal subgenomic RNAs that will generate the capsid and movement protein.
  7. Virus assembly in the cytoplasm.
  8. Viral movement protein probably mediates virion cell-to-cell transfer.

Host-virus interaction

Suppression of RNA silencing

Viruses of the Tombusviridae family encode proteins acting as suppressors of RNA silencing. Examples are tombusvirus P19/P20 , aureusvirus P14 , dianthovirus movement protein , carmovirus capsid protein P38 .

Matching UniProtKB/Swiss-Prot entries

(all links/actions below point to uniprot.org website)

99 entries grouped by protein

23 entries

Major capsid protein (Coat protein) (CP)

Select_allDeselect_all
CAPSD_AMCV
Artichoke mottled crinkle virus (AMCV) reference strain

Capsid protein (Coat protein) (p41)

CAPSD_BYDV1
Barley yellow dwarf virus (isolate MAV-PS1) (BYDV) reference strain
CAPSD_BYDVP
Barley yellow dwarf virus (isolate PAV) (BYDV) reference strain
CAPSD_CARMS
Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain

Capsid protein (Coat protein) (p38)

CAPSD_CARMV
Carnation mottle virus (CarMV) reference strain

Capsid protein (Coat protein) (p38)

CAPSD_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

Capsid protein (Coat protein) (p25)

CAPSD_MNSV
Melon necrotic spot virus (MNSV) reference strain

Capsid protein (Coat protein) (p42)

CAPSD_OCSVU
Oat chlorotic stunt virus (isolate United Kingdom) (OCSV) reference strain

Capsid protein (p48)

CAPSD_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

Capsid protein (Coat protein) (p26)

CAPSD_POLVP
Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

Capsid protein (p40)

CAPSD_RCNMV
Red clover necrotic mosaic virus (RCNMV) reference strain

Capsid protein (Coat protein)

CAPSD_TBSVC
Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain

Capsid protein (Coat protein) (p41)

CAPSD_TCV
Turnip crinkle virus (TCV) reference strain

Capsid protein (Coat protein) (p38)

CAPSD_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain

Capsid protein (p30)

CAPSD_BLRV
Bean leafroll virus (BLRV)
CAPSD_BYDVM
Barley yellow dwarf virus (isolate MAV) (BYDV)
CAPSD_BYDVN
Barley yellow dwarf virus (isolate NY-RPV) (BYDV)
CAPSD_BYDVR
Barley yellow dwarf virus (isolate P-PAV) (BYDV)
CAPSD_CNV
Cucumber necrosis virus (CNV)

Capsid protein (Coat protein) (p41)

CAPSD_CRSVL
Carnation ringspot virus (isolate Lommel) (CRSV)

Capsid protein (Coat protein)

CAPSD_CRV
Cymbidium ringspot virus (CymRSV)

Capsid protein (Coat protein) (p41)

CAPSD_TBSVB
Tomato bushy stunt virus (strain BS-3) (TBSV)

Capsid protein (Coat protein) (p41)

CAPSD_TNVD
Tobacco necrosis virus (strain D) (TNV-D)

Capsid protein (p30)

1 entry

Readthrough protein P3-RTD (P3-P5 readthrough protein) (P72) (Readthrough protein) (RT protein)

MCAPS_BYDVP
Barley yellow dwarf virus (isolate PAV) (BYDV) reference strain

2 entries

Movement protein (MP)

Select_allDeselect_all
MP_RCNMV
Red clover necrotic mosaic virus (RCNMV) reference strain
MP_CRSVL
Carnation ringspot virus (isolate Lommel) (CRSV)

5 entries

Double gene block protein 1 (DGBp1) (Movement protein P7)

Select_allDeselect_all
MP1_CARMS
Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain
MP1_CARMV
Carnation mottle virus (CarMV) reference strain
MP1_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

Protein p31

MP1_MNSV
Melon necrotic spot virus (MNSV) reference strain

Double gene block protein 1 (DGBp1) (Movement protein P7A)

MP1_TCV
Turnip crinkle virus (TCV) reference strain

Double gene block protein 1 (DGBp1) (Movement protein P8)

4 entries

Double gene block protein 2 (DGBp2) (Movement protein P9)

Select_allDeselect_all
MP2_CARMS
Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain
MP2_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

p7b

MP2_MNSV
Melon necrotic spot virus (MNSV) reference strain

Double gene block protein 2 (DGBp2) (Movement protein P7B)

MP2_TCV
Turnip crinkle virus (TCV) reference strain

2 entries

Movement protein p22 (p21)

Select_allDeselect_all
MP22_TBSVK
Tomato bushy stunt virus (strain Ja9) (TBSV)
MP22_TBSVT
Tomato bushy stunt virus (strain type) (TBSV)

1 entry

Putative movement protein p6.6

MP6_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

3 entries

Probable movement protein p8

Select_allDeselect_all
MP8_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain
MP8_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain
MP8_TNVD
Tobacco necrosis virus (strain D) (TNV-D)

15 entries

Movement protein (p22)

Select_allDeselect_all
MVP_BYDV1
Barley yellow dwarf virus (isolate MAV-PS1) (BYDV) reference strain

Movement protein (MP) (17 kDa protein)

MVP_BYDVP
Barley yellow dwarf virus (isolate PAV) (BYDV) reference strain

Movement protein (MP) (17 kDa protein)

MVP_GRVMC
Groundnut rosette virus (strain MC1) (GRV) reference strain

Movement protein

MVP_POLVP
Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

Movement protein (p27)

MVP_TBSVC
Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain
MVP_AMCV
Artichoke mottled crinkle virus (AMCV)
MVP_BLRV
Bean leafroll virus (BLRV)

Movement protein (MP) (16 kDa protein)

MVP_BYDVN
Barley yellow dwarf virus (isolate NY-RPV) (BYDV)

Movement protein (MP) (17 kDa protein)

MVP_BYDVR
Barley yellow dwarf virus (isolate P-PAV) (BYDV)

Movement protein (MP) (17 kDa protein)

MVP_CNV
Cucumber necrosis virus (CNV)
MVP_CRV
Cymbidium ringspot virus (CymRSV)
MVP_TBSV8
Tomato bushy stunt virus (strain B8) (TBSV)
MVP_TBSVA
Tomato bushy stunt virus (strain A23) (TBSV)
MVP_TBSVB
Tomato bushy stunt virus (strain BS-3) (TBSV)
MVP_TBSVJ
Tomato bushy stunt virus (strain Ja6) (TBSV)

1 entry

Long-distance movement protein (ORF3 protein)

ORF3_GRVMC
Groundnut rosette virus (strain MC1) (GRV) reference strain

1 entry

Uncharacterized protein ORF6

ORF6_BYDVP
Barley yellow dwarf virus (isolate PAV) (BYDV) reference strain

1 entry

Uncharacterized protein ORF7

ORF7_BYDVP
Barley yellow dwarf virus (isolate PAV) (BYDV) reference strain

1 entry

RNA silencing suppressor p14

P14_POLVP
Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

1 entry

Protein p15

P15_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

14 entries

RNA silencing suppressor p19 (19 kDa symptom severity modulator)

Select_allDeselect_all
P19_TBSVC
Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain
P19_AMCV
Artichoke mottled crinkle virus (AMCV)
P19_CIRV
Carnation Italian ringspot virus (CIRV)
P19_CNV
Cucumber necrosis virus (CNV)
P19_CRV
Cymbidium ringspot virus (CymRSV)
P19_HARV
Havel river virus (HaRV)
P19_PENSV
Pelargonium necrotic spot virus (PeNSV)
P19_PLV
Pear latent virus (PeLV)
P19_TBSV8
Tomato bushy stunt virus (strain B8) (TBSV)
P19_TBSVA
Tomato bushy stunt virus (strain A23) (TBSV)
P19_TBSVB
Tomato bushy stunt virus (strain BS-3) (TBSV)
P19_TBSVJ
Tomato bushy stunt virus (strain Ja6) (TBSV)
P19_TBSVK
Tomato bushy stunt virus (strain Ja9) (TBSV)
P19_TBSVT
Tomato bushy stunt virus (strain type) (TBSV)

1 entry

Uncharacterized protein p32

P32_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

2 entries

Uncharacterized protein p6

Select_allDeselect_all
P6_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain
P6_TNVD
Tobacco necrosis virus (strain D) (TNV-D)

1 entry

Uncharacterized protein p7

P7_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain

1 entry

Uncharacterized protein p8

P8_OCSVU
Oat chlorotic stunt virus (isolate United Kingdom) (OCSV) reference strain

19 entries

Putative RNA-directed RNA polymerase (EC 2.7.7.48)

Select_allDeselect_all
RDRP_BYDV1
Barley yellow dwarf virus (isolate MAV-PS1) (BYDV) reference strain
RDRP_BYDVP
Barley yellow dwarf virus (isolate PAV) (BYDV) reference strain
RDRP_CARMS
Carnation mottle virus (isolate China/Shanghai) (CarMV) reference strain

Probable RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_CARMV
Carnation mottle virus (CarMV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_GRVMC
Groundnut rosette virus (strain MC1) (GRV) reference strain

RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48)

RDRP_MCMVK
Maize chlorotic mottle virus (isolate United States/Kansas/1987) (MCMV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (111 kDa protein) (Protein p111)

RDRP_MNSV
Melon necrotic spot virus (MNSV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (p89)

RDRP_OCSVU
Oat chlorotic stunt virus (isolate United Kingdom) (OCSV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84)

RDRP_PMVK
Panicum mosaic virus (strain United States/Kansas 109S) (PMV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p112)

RDRP_POLVP
Pothos latent virus (isolate Pigeonpea/India) (PoLV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p84)

RDRP_RCNMV
Red clover necrotic mosaic virus (RCNMV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_TBSVC
Tomato bushy stunt virus (strain Cherry) (TBSV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

RDRP_TCV
Turnip crinkle virus (TCV) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_TNVA
Tobacco necrosis virus (strain A) (TNV-A) reference strain

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82)

RDRP_BYDVR
Barley yellow dwarf virus (isolate P-PAV) (BYDV)
RDRP_CNV
Cucumber necrosis virus (CNV)

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

RDRP_CRSVL
Carnation ringspot virus (isolate Lommel) (CRSV)

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p88)

RDRP_CRV
Cymbidium ringspot virus (CymRSV)

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p92)

RDRP_TNVD
Tobacco necrosis virus (strain D) (TNV-D)

RNA-directed RNA polymerase (EC 2.7.7.48) (Protein p82)